MA Wen-fang, TANG Yu-rong, XIE Feng-feng, et al. HPLC Fingerprint of [J]. Chinese journal of experimental traditional medical formulae, 2016, 22(10): 76-79.
DOI:
MA Wen-fang, TANG Yu-rong, XIE Feng-feng, et al. HPLC Fingerprint of [J]. Chinese journal of experimental traditional medical formulae, 2016, 22(10): 76-79. DOI: 10.13422/j.cnki.syfjx.2016100076.
Objective: To establish the HPLC fingerprint chromatogram of Crassocephalum crepidioides coupled with chemometrics means for the quality control of multi-batches of medicinal material. Method: The separation was developed on C18 column (Phenomenex
4.6 mm×250 mm
5 μm) by gradient elution with acetonitrile-water (both containing 0.1% phosphoric acid) as mobile phase at a flow rate of 1.0 mL·min-1
the detection wavelength at 328 nm and column temperature at 30℃. The HPLC fingerprint chromatogram of C. crepidioides was set up. The quality of 13 batches of medicinal material was evaluated by similarity assay as well as cluster analysis. Result: The establish HPLC fingerprint chromatogram of C. crepidioides was specfic
precise
reproduccible and stable. The similarities of 13 batches of C. crepidioides were more than 0.97. Further cluster analysis was carried out for the quality consistency of 13 batches of C. crepidioides and accordingly they were devided into 4 clusters. Conclusion: With the cluster analysis
the HPLC fingerprint chromatogram C. crepidioides provides a method for qulity control of C. crepidioides.
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Related Author
ZHANG Lin
ZHOU Fu-jun
SHAN Qi
GUO Qing-ling
HOU Wen-bin
Yan MENG
Wu-yi LI
Jia-ming SHAN
Related Institution
Key Laboratory of Modern Preparation of Traditional Chinese Medicine (TCM),Ministry of Education,Jiangxi University of TCM
Kunming Municipal Hospital of Traditional Chinese Medicine
Yunnan Provincial Key Laboratory of Research on External Drug Delivery System and Preparation Technology in University,Yunnan University of Chinese Medicine
College of Pharmacy and Chemistry, Key Laboratory of Universities in Yunnan Province on Development of Genuine Medicinal Materials Resources in Western Yunnan, Dali University