Objective: A high performance liquid chromatographic method with gradient elution and three wavelength detection was developed for the simultaneous determination of amygdalin

liquiritin and naringin in Juhong Huatan Wan. Method: The separation was performed on a Diamonsil-C18 column with methanol and 0.1% phosphoric acid as the mobile phase of gradient elution at a flow rate of 1.0 mL·min-1. The detection wavelengths were set at 210

276

283 nm. Result: The linear range of amygdalin was 25.0-175.0 mg·L-1(r=0.999 1). The average recovery for amygdalin was 101.5%

precision of the method was 1.9%(RSD). The linear range of liquiritin was 2.0-50.0 mg·L-1(r=0.999 5). The average recovery for liquiritin was 100.8%

precision of the method was 1.5%(RSD). The linear range of naringin was 6.3-126.0 mg·L-1(r=0.999 2). The average recovery for naringin was 101.7%

precision of the method was 1.6%(RSD). Conclusion: The method is simple

accurate and reproducible. It is a good method for the quality control of the preparation.