Objective: To discuss the effects of the total flavones from Mappianthus iodoies on myocardial ischemic-reperfused injury in rats and lay the foundation for clinical medicinal research of M. iodoies in the cardiovascular field. Method: Seventy-two male SD rats were randomly divided into six groups:sham-operation group

model group

positive drug group (compound Danshen dripping pills)

M. iodoies total flavones low dose group (60 mg·kg-1)

middle dose group (120 mg·kg-1) and high dose group (240 mg·kg-1). Six groups of rats received corresponding drugs for 10 days by intragastric administration. The ischemia-reperfusion model was induced by 30 min of coronary occlusion followed by 24 h of reperfusion in rats. Blood and heart were taken from the successfully modeled rats. Pathological changes of the cardiac tissues of rats were observed by hematoxylin-eosin(HE) staining;centrifugal treatment of the blood from femoral artery was done to obtain the supernatant. The levels of creatine kinase (CK) and lactic dehydrogenase (LDH) in serum were detected by enzyme-linked immunosorbent assay(ELISA). The levels of B-cell lymphoma/leukemia-2(Bcl-2) and Bcl-2 associated X protein(Bax) protein expressions were detected by Western blot. Result: As compared with the sham-operation group

the levels of CK and LDH in serum of rats in model group were significantly increased;Bcl-2 protein expression was reduced while Bax protein expression was increased (P < 0.05)

with obvious pathological morphology damages in myocardial tissues. As compared with the model group

the levels of CK and LDH were significantly reduced in serum of rats in M. iodoies total flavones all dose groups;Bax protein expression was significantly reduced while Bcl-2 protein expression was increased (P < 0.05)

with significant improvement in pathological morphology damages in myocardial tissues. Conclusion: The total flavones of M. iodoies have certain protective effect in rats with myocardial ischemic reperfused injury

and the mechanism may be related to the inhibition of cell apoptosis.