Objective: To investigate the effect of the Mongolian Zhurihen drop pills on ischemia/reperfusion injury and coagulation function. Method: The Na2S2O4-based hypoxia/reoxygenation injury models were established by H9c2 cells in vitro. The effects of Zhurihen drop pills on H9c2 cells viability

superoxide dismutase (SOD) activity

and malondialdehyde (MDA) activity were observed. Wistar rats were randomly divided into 6 groups:normal group;model group;positive drug group (Danshen drop pill group 72.9 mg·kg-1); Zhurihen drop pills low

middle

high dose groups (248

744

1 240 mg·kg-1). The hearts of rats were isolated and perfused with Langendorff apparatus. Except the rats of normal group

all the other rats were reperfused for 30 min after 30 min of global ischemia

resulting in the myocardial ischemia-reperfusion injury (I/R) models. Balloon catheter was inserted into the left atrium

then myocardial hemodynamic parameters were monitored and recorded

and the biochemical parameters such as lactate dehydrogenase (LDH) and creatine kinase (CK) in perfusate were measured as well. 40 healthy rabbits were divided into normal group

positive control group (Danshen pill group 37.8 mg·kg-1);Zhurihen drop pills low

middle

high dose groups (129

387

645 mg·kg-1) (n=8 in each group). After administration for 10 consecutive days

prothrombin time (PT)

activated partial thromboplastin time (APTT)

thrombin time (TT) and fibrinogen (FIB) were measured with four channel coagulation analyzer. Result: Compared with the model group

H9c2 cells viability was significantly increased (P < 0.01

P < 0.05)

SOD activity improved

and MDA content was decreased (P < 0.05) by the Zhurihen drop pills medicated serum. After I/R for 30 min

compared with the normal group

the rats in model group showed significant decline in left ventricular systolic pressure (LVSP) and positive and negative maximal values of the first derivative of left ventricular pressure (±dp/dtmax)(P < 0.01

P < 0.05)

significant increase in left ventricular end diastolic pressure (LVDP)(P < 0.01)

significant increase in levels of LDH and CK in perfusate(P < 0.01). Zhurihen drop pills low

middle

and high dose groups could significantly increase LVSP and ±dp/dtmax levels

significantly reduce LVDP level

LDH and CK content in perfusate (P < 0.01

P < 0.05). Conclusion: Zhurihen drop pills can protect cardiomyocyte against hypoxia/reoxygenation injury

improve heart function impairment

protect cardiac tissues

and prolong the coagulation time with anticoagulant effects.