Spectrum-effect Relationship of Petroleum Ether Fraction of Salt-processed Alpiniae Oxyphyllae Fructus on Arresting Polyuria

GONG Xiao-meng; WU Shan-shan; LI Meng-qi; ZHANG Mei; HU Qi; HU Chang-jiang

doi:10.13422/j.cnki.syfjx.2016110001

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Spectrum-effect Relationship of Petroleum Ether Fraction of Salt-processed Alpiniae Oxyphyllae Fructus on Arresting Polyuria

更新时间：2024-01-04

- Spectrum-effect Relationship of Petroleum Ether Fraction of Salt-processed Alpiniae Oxyphyllae Fructus on Arresting Polyuria
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 11, Pages: 1-5(2016)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2016110001
  CLC： R245
- Published：2016
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GONG Xiao-meng, WU Shan-shan, LI Meng-qi, et al. Spectrum-effect Relationship of Petroleum Ether Fraction of Salt-processed Alpiniae Oxyphyllae Fructus on Arresting Polyuria[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(11): 1-5.
DOI：

GONG Xiao-meng, WU Shan-shan, LI Meng-qi, et al. Spectrum-effect Relationship of Petroleum Ether Fraction of Salt-processed Alpiniae Oxyphyllae Fructus on Arresting Polyuria[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(11): 1-5. DOI： 10.13422/j.cnki.syfjx.2016110001.

摘要

目的: 通过盐炙益智仁石油醚部位的指纹图谱特征峰所代表的化学成分及其对豚鼠膀胱逼尿肌抑制作用贡献的大小

阐明盐炙益智仁的药效物质基础。方法: 采用HPLC建立盐炙益智仁石油醚部位的指纹图谱

Diamonsil C18色谱柱(4.6 mm×250 mm

5 μm)

流动相水(A)-乙腈(B)梯度洗脱(0~10 min

5%~25%B;10~60 min

25%~65%B;60~75 min

65%~100%B)

柱温35℃

检测波长254 nm

进样量10 μL

流速1 mL·min-1。考察盐炙益智仁石油醚部位对豚鼠离体膀胱逼尿肌活动的影响

分析盐炙益智仁缩尿药效的物质基础

利用灰色关联分析法建立谱效关系。结果: 建立了盐炙益智仁石油醚部位的指纹图谱

相似度均 > 0.9;盐炙益智仁缩尿的药效是多种成分共同作用的结果

各特征峰所代表的化学成分缩尿药效贡献的大小顺序(按特征共有峰编号)为20号 > 9号 > 11号 > 17号 > 16号 > 13号 > 15号 > 24号 > 18号 > 23号 > 14号 > 32号 > 34号 > 22号 > 25号 > 19号 > 31号 > 27号 > 21号 > 29号 > 28号 > 30号。结论: 构建的盐炙益智仁石油醚部位HPLC指纹图谱重复性好、稳定性高、方法简单

石油醚部位指纹图谱与其缩尿药效之间有较明显的对应关系

为盐炙益智仁的缩尿药效评价提供参考。

Abstract

Objective: To clarify pharmacodynamic material basis of salt-processed Alpiniae Oxyphyllae Fructus by investigating the contribution of chemical components from petroleum ether fraction of salt-processed products to the effect on detrusor in bladder of Guinea pig. Method: The fingerprint of petroleum ether fraction of salt-processed Alpiniae Oxyphyllae Fructus was established by HPLC

the mobile phase consisted of water and acetonitrile for gradient elution. Effect of petroleum ether fraction on detrusor in bladder of Guinea pig was investigated

and its material basis was analyzed

grey relational analysis method was used to establish the relationship between spectrum and efficacy. Result: The fingerprint of petroleum ether fraction of salt-processed products was established with similarity > 0.9. Detrusor inhibitory effect was the result of many kinds of components

the contribution of different peaks to relieve polyuria was ranked as 20 > 9 > 11 > 17 > 16 > 13 > 15 > 24 > 18 > 23 > 14 > 32 > 34 > 22 > 25 > 19 > 31 > 27 > 21 > 29 > 28 > 30. Conclusion: The HPLC fingerprints is simple and repeatable. The relationship between fingerprints of petroleum ether fraction and efficacy is paralleled to some extent

and this study will lay a foundation for the further research on inhibitory of salt-processed Alpiniae Oxyphyllae Fructus for polyuria.

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