Objective: To explore a decalcification method with easier operation and shorter time. Method: Forty DBA/1 mice were randomly divided into normal group and model group. Mice in model group were induced with type II collagen. Ankles were achieved from both model group and normal group

and each of groups were demineralized by improved method and conventional method

respectively. Effect of these two decalcification methods was compared from three aspects

including joint pathology

pathological scores and immunohistochemistry. Result: When the temperature was 4℃ and the replacement cycle of decalcification fluid was 24 h

the improved decalcification speed was 7 d

and this operation did not have an obvious effect on the structure and the protein expression of bone tissue. Conclusion: This optimized decalcification method can effectively improve the decalcification speed

which can get pathological diagnosis rapidly.