XIAO Chao-yong, ZHANG Yu, WANG Yu-liang. Extraction and Purification of Total Polysaccharides in Taraxaci Herba and Its Anti-inflammatory Activity[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(11): 25-28.
DOI:
XIAO Chao-yong, ZHANG Yu, WANG Yu-liang. Extraction and Purification of Total Polysaccharides in Taraxaci Herba and Its Anti-inflammatory Activity[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(11): 25-28. DOI: 10.13422/j.cnki.syfjx.2016110025.
Extraction and Purification of Total Polysaccharides in Taraxaci Herba and Its Anti-inflammatory Activity
Objective: To study on total polysaccharides in Taraxaci Herba and its in vitro anti-inflammatory activity for screening active ingredient of anti-inflammatory. Method: Water extract-alcohol precipitation method was adopted to gain total polysaccharides in Taraxaci Herba (TMP)
and then it was purified by DEAE cellulose column to get three parts of TMP (TMP-0
TMP-1
TMP-2). Anti-inflammatory activity of TMP on RAW264.7 cells induced by LPS was investigated. Result: TMP can significantly reduce the mRNA expression of inflammatory factors [cyclooxygenase-2 (COX-2)
tumor necrosis factor-α (TNF-α)
interleukin-6 (IL-6) and interleukin-1β(IL-1β)] in RAW264.7 cells with a dose-dependent manner. The two fractions
TMP-0 and TMP-1
can significantly reduce the mRNA of these above inflammatory factors
they showed a good in vitro anti-inflammatory activity
while the anti-inflammatory effect of TMP-2 at low and middle doses was not obvious. Conclusion: TMP has a strong anti-inflammatory activity
and its main active fractions are TMP-0 and TMP-1.
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