LIN Ying-ying, XIONG Yong-ai, DAI Liang-min, et al. Optimization of Supercritical CO Extraction and Ethanol Extraction Processes of Yolk Oil[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(11): 29-33.
DOI:
LIN Ying-ying, XIONG Yong-ai, DAI Liang-min, et al. Optimization of Supercritical CO Extraction and Ethanol Extraction Processes of Yolk Oil[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(11): 29-33. DOI: 10.13422/j.cnki.syfjx.2016110029.
Optimization of Supercritical CO Extraction and Ethanol Extraction Processes of Yolk Oil
Objective: To optimize the conditions of supercritical CO2 extraction and ethanol extraction processes of yolk oil. Method: HPLC-ELSD was employed to determine the content of phosphatidylcholine with mobile phase A of methanol-water-glacial acetic acid-triethylamine (85: 15: 0.45: 0.05) and mobile phase B of n-hexane-isopropanol-mobile phase A (20: 48: 32) for gradient elution. Based on single factor tests
taking yield of yolk oil and the content of phosphatidylcholine as indexes
orthogonal tests were adopted to optimize supercritical CO2 extraction and ethanol extraction processes of yolk oil. Result: Optimum extraction conditions were as follows
moisture content of yolk oil was ≤5%
supercritical CO2 extraction temperature was 50℃
pressure was 35 MPa
flow was 50 L·h-1
extraction time was 2 h
yield of yolk oil was 42.65%
residual powder of supercritical CO2 extraction was extracted four times with four times the amount of 90% ethanol for 1.5 h of each time
the content of phosphatidylcholine was up to 17.24%. Conclusion: These optimized technologies are stable and feasible for development and application of yolk oil.
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