Optimization of Extraction Technology for Total Nucleosides from  by Orthogomal Test

ZHANG Jing; DING Bo; ZHANG Hua; QI Jun-sheng; ZHOU Nong; CHEN Xiu-hong; LIU Luo

doi:10.13422/j.cnki.syfjx.2016110034

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Optimization of Extraction Technology for Total Nucleosides from by Orthogomal Test

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- Optimization of Extraction Technology for Total Nucleosides from by Orthogomal Test
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 11, Pages: 34-37(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016110034
  CLC： R284.2
- Published：2016
- 稿件说明：
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ZHANG Jing, DING Bo, ZHANG Hua, et al. Optimization of Extraction Technology for Total Nucleosides from by Orthogomal Test[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(11): 34-37.
DOI：

ZHANG Jing, DING Bo, ZHANG Hua, et al. Optimization of Extraction Technology for Total Nucleosides from by Orthogomal Test[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(11): 34-37. DOI： 10.13422/j.cnki.syfjx.2016110034.

摘要

目的: 优选川党参中核苷类成分的提取工艺条件

为该有效部位的开发提供基础资料。方法: 采用超声波提取川党参中核苷类成分

以胞苷、尿苷、鸟苷、腺苷的总提取量为评价指标

在单因素试验基础上

通过正交试验考察不同因素对川党参中核苷类成分提取量的影响。运用HPLC测定胞苷、尿苷、鸟苷、腺苷的含量

流动相甲醇(A)-水(B)梯度洗脱(0~10 min

1%~5%A;10~15 min

5%~15%A;15~22 min

15%~17%A;22~26 min

17%~20%A;26~32 min

20%~24%A)

检测波长260 nm。结果: 各因素对核苷类成分提取量的影响顺序为甲醇体积分数 > 提取次数 > 料液比 > 提取时间

最佳提取工艺为加10倍量水提取2次

每次30 min

川党参中4种核苷类成分总提取量1.523 mg·g-1。结论: 建立的HPLC检测川党参中核苷类成分的含量方法准确可靠。优选的提取工艺稳定可行

具有节约成本、快速、高效等优点。

Abstract

Objective: To optimize extraction conditions of total nucleosides in Codonopsis tangshen. Method: Ultrasonic method was adopted to extracting total nucleosides in C. tangshen. Taking the contents of total nucleosides (cytidine

uridine

guanosine and adenosine) as index

based on single factor tests

orthogonal test was designed to optimize extraction conditions of total nucleosides in C. tangshen. HPLC was employed to determine the contents of cytidine

uridine

guanosine and adenosine with mobile phase of methanol-water for gradient elution and detection wavelength at 260 nm. Result: The influence of various factors on the extraction rate of total nucleosides was on order of volume fraction of methanol > extraction times > solid-liquid ratio > extraction time. Optimal parameters was as follows:extracted twice with 10 times the amount of water for 30 min of each time. Under these conditions

extracting amount of total nucleosides was 1.523 mg·g-1. Conclusion: This established HPLC method is accurate and reliable to detect contents of total nucleosides. At the same time

this optimized extraction technology is stable and feasible with the advantages of energy-saving

rapidity

efficient and so on.

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