Simultaneous Determination of Four Constituents in  by RP-HPLC

WEI Min; SUN Xuan

doi:10.13422/j.cnki.syfjx.2016110077

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Simultaneous Determination of Four Constituents in by RP-HPLC

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- Simultaneous Determination of Four Constituents in by RP-HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 11, Pages: 77-79(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016110077
  CLC： O657.72;R29
- Published：2016
- 稿件说明：
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WEI Min, SUN Xuan. Simultaneous Determination of Four Constituents in by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(11): 77-79.
DOI：

WEI Min, SUN Xuan. Simultaneous Determination of Four Constituents in by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(11): 77-79. DOI： 10.13422/j.cnki.syfjx.2016110077.

摘要

目的: 建立维吾尔族药材硬尖神香草中槲皮素、咖啡酸、木犀草素、金合欢素4种成分的含量测定方法。方法: 使用Agilent C18色谱柱(4.6 mm×250 mm

5 μm)

流动相甲醇-0.2%乙酸水溶液(30:70)

检测波长340 nm

流速1.0 mL·min-1

柱温30℃

进样量10 μL。结果: 槲皮素、咖啡酸、木犀草素、金合欢素分别在0.120 2~1.081 8 mg(r=0.998 6)

0.031~0.279 mg (r=0.999 9)

0.052~0.468 mg(r=0.998 9)

0.098 1~0.882 9 mg(r=0.998 8)呈良好的线性关系

回收率分别为98.62%(RSD 1.4%)

97.38%(RSD 1.7%)

99.41%(RSD 1.5%)

99.81%(RSD 1.0%)

结论: 采用RP-HPLC同时测定硬尖神香草中4种成分含量方法

该方法可行且简单有效

可作为硬尖神香草含量测定的理论指标。

Abstract

Objective: To establish a method for determining the contents of quercetin

caffeic acid

luteolin

and acacetin in Uygur Medicine Hyssopus cuspidatus. Method: Agilent C18 chromatographic column (4.6 mm×250 mm

5 μm) was used with methanol-0.2% acetic acid solution as the mobile phase. The detection wavelength was set at 340 nm and the flow rate of 1.0 mL·min-1. The column temperature was 30℃ and the injection volume was 10 μL. Result: Quercetin

caffeic acid

luteolin

and acacetin showed good linear relationship in 0.120 2-1.081 8 mg(r=0.998 6)

0.031-0.279 mg(r=0.999 9)

0.052-0.468 mg(r=0.998 9)

and 0.098 1-0.882 9 mg(r=0.998 8) respectively

and their recovery rate was 98.62%(RSD 1.4%)

97.38%(RSD 1.7%)

99.41%(RSD 1.5%)

and 99.81%(RSD 1.0%) respectively. Conclusion: This RP-HPLC method is feasible and simple for simultaneous determination of 4 components in H. cuspidatus

which can provide theoretical indicators for the content determination of H. cuspidatus.

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Related Author

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Related Institution

Henan Engineering Research Center for Quality Control and Evaluation of Traditional Chinese Medicine（TCM），School of Pharmacy，Henan University of Chinese Medicine

Luohe Medical College

Third Affiliated Hospital of Henan University of Chinese Medicine

Tianjin University of Traditional Chinese Medicine

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

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