Objective: To establish a quantitative analysis of multi-components with a single-marker (QAMS) method for the determination of five kinds of water-soluble components in Aconiti Lateralis Radix Praepapata. Method: With hydrochloride dopamine as the reference

chromatographic separation was achieved on a Diamonsil C18 column (4.6 mm×250 mm

5 μm). The content in five kinds of water-soluble components of aconite was determined and elative correction factors were calculated with methanol-0.2% phosphoric acid water as the mobile phase with gradient elution

at column temperature of 30℃ and flow rate of 1.0 mL·min-1

with detection wavelength of 260 nm and 280 nm. External standard method and QAMS assay were used to respectively determine the contents in 10 batches of aconite medicinal materials and verify the accuracy and feasibility of QAMS. Result: QAMS was established for determining five kinds of water-soluble components of in Aconiti Lateralis Radix Praepapata

and 10 batches of aconite were measured. The difference between the calculated and measured values was small (RSD < 5%). Conclusion: QAMS for measure five kinds of water-soluble components in Aconiti Lateralis Radix Praepapata is simple

effective

accurate

and provides a reference for its subsequent evaluation study.