Objective: To establish an HPLC-ELSD method for simultaneous determination of ginsenosides Rg1

Rb1

Rd and astragaloside Ⅳ in Yixin Xuezhikang capsules. Method: Shimadzu C18 (4.6 mm×150 mm

5 μm) was used

with acetonitrile-water as the mobile phase for gradient elution. The column temperature was 35℃

with a flow rate of 1.0 mL·min-1. The temperature of ELSD drift tube was set at 60℃

temperature of atomizing chamber at 30℃ and the gas pressure was 23.0 psi. Result: Excellent chromatographic separation was achieved for four active compounds (ginsenosides Rg1

Rb1

Rd

and astragaloside Ⅳ) with good linearity (r≥0.999 1) within the measurement range between mass concentration and peak area. The average recovery rate was 99.60% (RSD 1.6%)

99.89% (RSD 2.8%)

98.13% (RSD 2.7%) and 98.36% (RSD 2.5%) respectively for these four compounds. Conclusion: The method is simple

accurate

reproducible

and can be used as the quality control method for Yixin Xuezhikang capsules.