YANG Jian-hua, WANG Xiao-mei, Dilibar·, et al. Study on Metabolites of Aesculin and Aesculetin in Rat Urine by HPLC-MS[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(11): 93-96.
DOI:
YANG Jian-hua, WANG Xiao-mei, Dilibar·, et al. Study on Metabolites of Aesculin and Aesculetin in Rat Urine by HPLC-MS[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(11): 93-96. DOI: 10.13422/j.cnki.syfjx.2016110093.
Study on Metabolites of Aesculin and Aesculetin in Rat Urine by HPLC-MS
Objective: To investigate the metabolites of aesculin and aesculetin in rat urine. Method: After single oral administration of aesculin and aesculetin to rats
urine was collected within 48 h and pretreated by solid-phase extraction method. Metabolites were determined by a HPLC-MSn method. Result: Five major metabolites were found and their chemical structures were elucidated as hydrolysate
reduzate
methylated metabolite and sulfated product
respectively. Conclusion: According to the rule of metabolism
aesculin is hydrolyzed as aesculetin which were further metabolized in vivo. The method can be used to identify the structures of active components responsible for pharmacological effects.
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