Objective: To observe the effect of extract from Ginseng Radix et Rhizoma

Notoginseng Radix et Rhizoma and Chuanxiong Rhizoma(EGNC) on the smooth muscle 22α (SM22α) expressions of replicative senescence vascular smooth muscle cells and cytoskeleton-associated protein. Method: With human aortic smooth muscle cells as the research objects and the replicative senescence 9th generation cells as the aging model

this experiment was divided into youth group (5th generation cells)

model group (9th generation cell)

EGNC low dose group (100 mg·L-1)

middle dose group (200 mg·L-1)

and high dose group (400 mg·L-1)

resveratrol group (10 μmol·L-1). The drug intervention time was 48 h. β-galactosidase staining method was used to calculate blue dye cell ratio. The cell cycle was analyzed by the flow cytometry. Immunofluorescent staining method was used to observe the morphological changes of SM22α;while the mRNA and protein expressions of SM22α were detected by qPCR and Western blot respectively. Result: Compared with the youth group

the cell size was increased and shape was irregular in model;number of cells in G0/G1 stage was increased and number of cells in S stage was reduced;number of blue-dyed cells in β-galactosidase staining method was reduced (P < 0.01)

conforming to the characteristics of aging model;in addition

SM22α staining was fuzzy and bleak

and the fluorescence intensity was weakened significantly

with reduced mRNA and protein expressions of SM22α (P < 0.01). As compared with the model group

drug intervention effectively reduced the number of blue-dyed cells

reduced the number of cells in G0/G1

increased the number of cells in S stage

enhanced the fluorescence staining intensity of SM22α

and increased mRNA and protein expressions of SM22α

with statistically significant difference (P < 0.05

P < 0.01). Conclusion: The replicative senescence vascular smooth muscle cells can be used as aging research models. The morphology

gene and protein expressions of SM22α were changed obviously in the process of cell aging

and it may involve in the process of cell aging together with cytoskeleton. EGNC delayed the aging of vascular smooth muscle cells to a certain extent and had obvious intervention effect on SM22α

so it might delay the aging of vessels with the cytoskeleton indirectly.