Objective: To investigate the clinical efficacy of Guishen pills for premature ovarian failure(POF) with syndrome of kidney-yin deficiency and observe its effect on levels of growth differentiation factor-9 (GDF-9) in serum and bone morphogenetic protein-15(BMP-15). Method: One hundred and sixteen POF patients were randomly divided into control group (58 cases) and comprehensive group (58 cases) by random number table. The patients of control group received conjugated estrogen tablets for 20 days (1 tablet/time

qd);medroxyprogesterone acetate tablets were added from day 16 to day 20 (4 tablets/time

qd). Based on the treatment of control group

patients in comprehensive group were also treated with Guishen pills (1 dose/d

bid

stop in menstrual period). 20 days were regarded as one treatment course

and the patients in both groups were treated for 3 courses. Traditional Chinese medicine(TCM) symptom scores

follicle-stimulating hormone (FSH)

estradiol (E2) and luteinizing hormone(LH) levels in serum were compared between two groups. The levels of GDF-9 and BMP-15 in serum were detected in two groups. Result: TCM symptom scores in comprehensive group were significantly lower than those in control group after treatment (P < 0.01). The total clinical effective rate was 94.83% in comprehensive group

significantly higher than 81.03% in control group(P < 0.05). The levels of FSH and LH in serum of comprehensive group were significantly lower than those of control group after treatment

while E2 level was significantly higher than that of control group (P < 0.01). The levels of GDF-9 and BMP-15 in serum of comprehensive group were significantly higher than those of control group

with statistically significant differences (P < 0.01). Conclusion: On the basis of routine western medicine treatment

Guishen pills could obviously improve clinical symptoms

hormonal levels

and clinical efficacy. The mechanism may be associated with increasing GDF-9 and BMP-15 levels in serum.