TAO Yong-hua, SUN Lan, YIN Hong-mei, et al. Optimization of Alcohol Precipitation Technology of Wubie Granules Intermediates Based on Pharmacodynamic Screening[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(12): 28-31.
DOI:
TAO Yong-hua, SUN Lan, YIN Hong-mei, et al. Optimization of Alcohol Precipitation Technology of Wubie Granules Intermediates Based on Pharmacodynamic Screening[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(12): 28-31. DOI: 10.13422/j.cnki.syfjx.2016120028.
Optimization of Alcohol Precipitation Technology of Wubie Granules Intermediates Based on Pharmacodynamic Screening
Objective: To optimize alcohol precipitation technology of Wubie granules intermediates for reducing the daily dose of this preparation. Method: The rationality of alcohol precipitation technology of Wubie granules intermediates was determined by investigating activities of estradiol (E2)
follicle stimulating hormone (FSH)
luteinizing hormone (LH) of ovariectomized rats and uterus
ovaries of mice. Taking transfer rates of stilbene glucoside
asperosaponin Ⅵ and solid content as indexes
single factor tests were adopted to optimize alcohol precipitation technology. Contents of stilbene glucoside and asperosaponin Ⅵ were determined by HPLC. Result: Optimum alcohol precipitation process was as follows:concentrated extracting liquid of Polygoni Multiflori Radix praeparata group to relative density of 1.10 (60℃)
added ethanol until the concentration of ethanol to 60%
standing time of >12 h
concentrated supernatant to relative density of 1.20-1.23 (60℃). Conclusion: Optimal alcohol precipitation technology is reasonable and reliable
which determines by pharmacodynamic screening and single-factor tests. This process can reduce the daily dose and ensure the effectiveness of Wubie granules.