Objective: To study the genes related to the synthesis of diterpenoid. Based on the data of transcriptome sequencing

cDNAs encoding 1-deoxy-D-xylulose-5-phosphatereduetoisomerase (DXR) were obtained from the leaves of aseptic seedlings of Rabdosiae Rubescentis Herba. Method: DXR was obtained by reverse transcription PCR. Real-time quantitative PCR was used to detect the relative expression patterns of DXR in different tissues of Rabdosiae Rubescentis Herba. Result: Sequence analysis showed that the full-length cDNA of DXR was 1 500 bp and contains gene open reading frame (ORF) of 1 422 bp encoding 473 amino acids. The theoretical molecular weight was 51.39 kDa and the isoelectric point was predicted as 6.09

suggesting it was a type of hydrophilic protein. The expression pattern of the gene in different tissues was analyzed by Real-time fluorescence quantitative PCR. The results showed the expression of DXR was relatively high in the stem and the lowest in callus. Conclusion: The results will provide a basis for studying the activity and function of DXR from Rabdosiae Rubescentis Herba

and lay a foundation for biosynthesis and gene mining of terpenoids.