ZHU Yi-lei, FANG Hai-hong, WEI Hui-zhen, et al. Determination of Three Alkaloids in XianglianPills with Ionic Liquids as Mobile Phase Additives[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(12): 75-78.
DOI:
ZHU Yi-lei, FANG Hai-hong, WEI Hui-zhen, et al. Determination of Three Alkaloids in XianglianPills with Ionic Liquids as Mobile Phase Additives[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(12): 75-78. DOI: 10.13422/j.cnki.syfjx.2016120075.
Determination of Three Alkaloids in XianglianPills with Ionic Liquids as Mobile Phase Additives
Objective: To establish a method to determine the contents of threealkaloids (berberine
jatrorrhizine and palmatine)in Xianglian pills with ionic liquids [BMIM]PF6 as mobile phase additives. Method: The effect of ionic liquid concentration was investigatedby using the Ultimate XB-C18(4.6 mm×250 mm
5 μm) column for separation
and compared with traditionaltriethylamine (TEA) additives then. Result: Ionic liquid added to the mobile phase couldimprove thepeak pattern of C18separative chromatographiccolumn
improve the separation degree
and the effect was better than that of traditional triethylamine additives. Conclusion: This method can be used for the simultaneous determination of a variety of alkaloids in Xianglian pills.