GC-MS Fingerprint of

LIU Bin; LIU Guo-liang; LI Yan-wei; MU Lu-lu; FANG Heng; ZHANG Ning; LIU Hai-yang

doi:10.13422/j.cnki.syfjx.2016130059

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GC-MS Fingerprint of

更新时间：2024-01-04

- GC-MS Fingerprint of
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 13, Pages: 59-62(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016130059
  CLC： O657.63;R284.1
- Published：2016
- 稿件说明：
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LIU Bin, LIU Guo-liang, LI Yan-wei, et al. GC-MS Fingerprint of [J]. Chinese journal of experimental traditional medical formulae, 2016, 22(13): 59-62.
DOI：

LIU Bin, LIU Guo-liang, LI Yan-wei, et al. GC-MS Fingerprint of [J]. Chinese journal of experimental traditional medical formulae, 2016, 22(13): 59-62. DOI： 10.13422/j.cnki.syfjx.2016130059.

摘要

目的: 建立肾茶挥发性成分的GC-MS指纹图谱。方法: 采用GC-MS结合程序升温保留指数技术对12批肾茶样品进行分析

DB-5MS色谱柱

载气氦气(He)

流速1 mL·min-1

进样量1μL

分流比100:1。质谱条件为电子轰击(EI)离子源

辅助线温度280℃

离子源230℃

四极杆150℃;质量扫描范围m/z 30~600。结果: 分析了12个批次的肾茶样品

建立了肾茶挥发性成分GC-MS的指纹图谱

鉴定了13个共有色谱峰成分。采用"中药色谱指纹图谱相似度评价系统软件"(2004 A版)进行指纹图谱分析

12批肾茶样品相似度均>0.999。结论: GC-MS结合程序升温保留指数技术能较为全面地反映肾茶挥发性成分

所建立的肾茶GC-MS指纹图谱特征性及专属性强

可作为肾茶挥发性成分质量控制的有效方法。

Abstract

Objective: To establish GC-MS fingerprint of Clerodendranthus spicatus volatile components. Method: Volatile components in twelve batches of C. spicatus samples were analyzed by GC-MS method combined with temperature-programmed retention index technique. Research was performed on DB-5MS chromatographic column; carrier gas was helium (He)

its flow rate was 1.0 mL·min-1

and the injection volume was 1 μL

the refractive ratio of distillation was 100:1.MS conditions were as follows:electron impact (EI) ion source

transmission line temperature of 280℃

ion source temperature of 230℃

quadrupole temperature of 150℃

and mass scan range of m/z 30-600. Result: Thirteen common peaks were identified in the GC-MS fingerprint of volatile components from 12 batches of C. spicatus samples. Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica(Version 2004 A) was used for fingerprint analysis. Similarity of 12 batches of C. spicatus samples was higher than 0.999. Conclusion: GC-MS combined with temperature-programmed retention index can more comprehensively reflect the volatile components of C.spicatus. The established GC-MS fingerprint is highly characteristic and specific

which can be used as an effective method for quality control of volatile components of C.spicatus.

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Related Institution

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

Shandong University of Traditional Chinese Medicine

Qilu University of Technology （Shandong Academy of Sciences）， Shandong Analysis and Test Center

2),LIU Dong-hui~1

广州中医药大学第一附属医院

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