SUN Hai-tao, HE Song-qi, WEN Bin, et al. Effects of Biejiajian Wan on Wnt Signal Pathway Molecules and Target Genes in HepG2 Cell Xenograft in Nude Mice[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(13): 81-85.
DOI:
SUN Hai-tao, HE Song-qi, WEN Bin, et al. Effects of Biejiajian Wan on Wnt Signal Pathway Molecules and Target Genes in HepG2 Cell Xenograft in Nude Mice[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(13): 81-85. DOI: 10.13422/j.cnki.syfjx.2016130081.
Effects of Biejiajian Wan on Wnt Signal Pathway Molecules and Target Genes in HepG2 Cell Xenograft in Nude Mice
Objective: To discuss the molecular mechanism of Biejiajian Wan in inhibiting hepatocellular carcinoma by studying its effect on the signaling molecules of Wnt/β-catenin signaling pathway and the expression of target genes on HepG2 cell xenograft in nude mice. Method: The hepatocellular carcinoma mode was established by tumor formation experiment in nude mice
and the mice were randomly divided into the high dose group (2.2 g·kg-1)
the middle dose group (1.1 g·kg-1)
the low dose group (0.55 g·kg-1)
the positive control group
and the normal control group. According to the visual observation
Biejiajian Wan had an inhibitory effect on the growth of implanted tumors of HepG2 cell xenograft in nude mice. Expressions of β-catenin and T-box 3 (TBX3) of HepG2 cell xenograft were measured by the immunohistochemical method. Expressions of p19ARF
murine double minute 2 (MDM2) and p53 in HepG2 cell xenograft were tested by Western blot. Result: Biejiajian Wan can significantly inhibit the growth of the implanted tumor of HepG2 cell xenograft in nude mice
and the expressions of β-catenin and TBX3 (P < 0.05)
as well as the expression of the p19ARF
p53 and MDM2 (P < 0.05). Conclusion: Biejiajian Wan can regulate the expression of β-catenin and TBX3 by affecting the Wnt/β-catenin signaling pathway
and then activate the cancer suppressor gene p53 by regulating the TBX3/p19ARF/MDM2 signaling pathway. Finally
Biejiajian Wan can repress the growth of liver cancer cells and induce cell apoptosis
Role and Mechanism of Cucurbitacin B in Suppressing Proliferation of Breast Cancer 4T1 Cells via Inducing Ferroptosis
Mechanism of Polyphyllin Ⅱ in Induction of Ferroptosis in Hepatocellular Carcinoma HepG2 Cells
Effect of Haizao Yuhutang Combined with Modified Sargassum and Glycyrrhizae Radix et Rhizoma on Expression of p53/p21/Caspase-3 Pathway in Rats with Goiter
Effect of Sargassum and Glycyrrhizae Radix et Rhizoma Incompatible Pair with Haizao Yuhutang on Oxidative Stress in Liver of Goiter Rats
Intervention Mechanism of Biejiajian Wan on Primary Liver Cancer by Regulating lncRNA SNHG5/miRNA-26a-5p/GSK-3β Signal Axis
Related Author
RUAN Yidan
ZHANG Huizhong
HUANG Huating
ZHANG Pingzhi
YAO Aina
ZHANG Yongqiang
XU Xiaohan
LI Shiman
Related Institution
School of Chinese Material Medica, Beijing University of Chinese Medicine
National Institutes for Food and Drug Control
School of Chinese Materia Medica, Beijing University of Chinese Medicine