LIANG Wei-hai, HUANG Peng, LI Lun, et al. Effects of Kuan Quzhen Gelatum on Eczema Model Rats[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(13): 97-101.
DOI:
LIANG Wei-hai, HUANG Peng, LI Lun, et al. Effects of Kuan Quzhen Gelatum on Eczema Model Rats[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(13): 97-101. DOI: 10.13422/j.cnki.syfjx.2016130097.
Effects of Kuan Quzhen Gelatum on Eczema Model Rats
Objective: To study and explore the effect and mechanism of Kuan Quzhen gelatum on rat model with eczema. Method: The eczema model of rat was established with 2
4-dinitrochlorobenzene. Totally 40 rats were divided into four groups
namely normal group
eczema group
positive control group and Kuan Quzhen gelatum group. The effect was observed by skin lesion score. Pathological changes were observed by paraffin sections. Interferon-γ(IFN-γ)
interleukin-4(IL-4)
interleukin-17 (IL-17)
interleukin-31 (IL-31)
leukotriene B4 (LTB4) and leukotriene C4 (LTC4) were measured by enzyme-linked immunosorbent assay. The expressions of cysteinyl leukotriene receptor 1 (CysLTR1) and cysteinyl leukotriene receptor 2 (CysLTR2) were detected by immunohistochemistry method and Western blot. Result: Compared with normal group
eczema model group showed hyperkeratosis
parakeratosis
hypergranulosis and acanthosis
with increases in IL-4
IL-17
IL-31
LTB4 and LTC4 and up-regulation in the expressions of CysLTR1 and CysLTR2.Compared with eczema model group
Kuan Quzhen Gelatum group showed reductions in skin lesion score after 6 days
pathological changes
and the level of IL-4
IL-17
IL-31 and LTC4 (P < 0.05)
and down-regulations in the expressions of CysLTR1 and CysLTR2
with no statistically significant difference in the level of LTB4. Conclusion: Kuan Quzhen Gelatum has therapeutic effects on rat model with eczema
which may be related with declines in IL-4
IL-17
IL-31 and LTC4 levels in serum and down-regulation in CysLTR1 and CysLTR2 expressions.
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