Objective: To study the effect of Guizhi Fuling capsule and its 15 active components on pelvic inflammation in rat models

and preliminary investigate its treatment effect for pelvic inflammation. Method: Totally 90 female SD rats were divided into sham-operation group and model group. The pelvic inflammatory models were established by Escherichia coli. The successfully modeled rats were further randomly divided into model group

Guizhi Fuling capsule group (250 mg·kg-1) and 15 active components low dose group

middle dose group and high dose group (75

150

300 mg·kg-1)

n=15 in each group. After intragastric administration for 15 d

the rats in Guizhi Fuling capsule group and active components groups were killed. Then their uterus was taken for weighing the uterus and calculating the uterus weight coefficient. Blood was obtained to measure tumour necrosis factor-α(TNF-α)

interleukin-2 (IL-2) levels in serum and immune globulinA (IgA)

IgG and IgM levels in plasma. Pathological changes of uterine tissues were also detected by htoxylin eosin (HE) staining method. Result: As compared with the sham-operation group

the uterus weight coefficient was significantly increased in rats of model group.IgA

IgG and IgM levels in plasma were significantly reduced

and uterine pathology composite score was significantly increased (P < 0.01). As compared with the model group

the uterus weight coefficient was significantly reduced in pelvic inflammatory rats of Guizhi Fuling capsule group and active components groups

TNF-αlevel in serum was significantly reduced;IL-2 level in serum was increased

the IgA

IgG and IgM levels in plasma were increased;and uterine pathology composite score was also significantly reduced (P < 0.05

P < 0.01). Conclusion: The 15 active components and Guizhi Fuling capsule have significant effects on anti-pelvic inflammatory disease in rats

indicating that the 15 active components may be the main effective ingredients of Guizhi Fuling capsule in the treatment of pelvic inflammatory disease.