Efficacy of Allicin for Non-alcoholic Fatty Liver and Effects on TNF-, SREBP-1c Protein Expression Levels in Model Rats

ZHOU Yuan-yuan; LIU Chun-jie; CHU Xian-xiang; LI Chao-yan

doi:10.13422/j.cnki.syfjx.2016130127

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Efficacy of Allicin for Non-alcoholic Fatty Liver and Effects on TNF-, SREBP-1c Protein Expression Levels in Model Rats

更新时间：2024-01-04

- Efficacy of Allicin for Non-alcoholic Fatty Liver and Effects on TNF-, SREBP-1c Protein Expression Levels in Model Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 13, Pages: 127-130(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016130127
  CLC： R285.5
- Published：2016
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ZHOU Yuan-yuan, LIU Chun-jie, CHU Xian-xiang, et al. Efficacy of Allicin for Non-alcoholic Fatty Liver and Effects on TNF-, SREBP-1c Protein Expression Levels in Model Rats[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(13): 127-130.
DOI：

ZHOU Yuan-yuan, LIU Chun-jie, CHU Xian-xiang, et al. Efficacy of Allicin for Non-alcoholic Fatty Liver and Effects on TNF-, SREBP-1c Protein Expression Levels in Model Rats[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(13): 127-130. DOI： 10.13422/j.cnki.syfjx.2016130127.

摘要

目的: 观察大蒜素对非酒精性脂肪性肝病(NAFLD)模型大鼠治疗作用及肝组织内肿瘤坏死因子-α(TNF-α)蛋白、固醇调节元件结合蛋白(SREBP-1c)蛋白的影响

并探讨其作用机制。方法: 76只雄性SD大鼠随机分为正常组(n=12)和造模组(n=64)

采用高脂饮食法诱导构建NAFLD大鼠模型

模型大鼠随机分为非诺贝特组(50 mg·kg-1·d-1)

模型组

大蒜素低、中、高剂量组(10

60 mg·kg-1·d-1)

每组12只。非诺贝特组及大蒜素组灌喂相应剂量药物

正常组和模型组灌喂等体积生理盐水。药物干预4周后处死动物

检测各组大鼠血清及肝组织内总胆固醇(TG)

甘油三酯(TC)

丙氨酸氨基转移酶(ALT)

天门冬氨酸氨基转移酶(AST)

苏木素-伊红(HE)染色观察肝组织病理变化

免疫组化检测肝组织中TNF-α

SREBP-1c蛋白表达。结果: 与正常组比较

模型组大鼠肝组织及血清TG

肝组织ALT

AST活性

肝组织中TNF-α

SREBP-1c蛋白表达均明显升高(P < 0.05

P < 0.01);与模型组比较

非诺贝特组、大蒜素低、中、高剂量组均明显降低大鼠肝组织及血清TG

肝组织ALT

AST活性

肝组织中TNF-α

SREBP-1c蛋白表达(P < 0.05

P < 0.01)结论: 大蒜素对高脂饮食诱导的NAFLD大鼠模型具有降脂护肝的作用

其机制与下调TNF-α及SREBP-1c有关。

Abstract

Objective: To observe the treatment efficacy of Allicin and effects on tumor necrosis factor-α(TNF-α)

sterol regulatoryelement binding protein-1c (SREBP-1c) protein levels in model rats of non-alcoholic fatty liver disease (NAFLD)

and to investigate the molecular mechanism. Method: seventy-six male SD rats were randomly divided into normal group (n=12) and model group (n=64). NAFLD models were established by high fat diet

and the rats in model group were further randomly divided into model group

fenofibrate group (50 mg·kg-1·d-1)

allicin low dose

middle dose and high dose groups (10

60 mg·kg-1·d-1)

n=12 in each group. The rats in fenofibrate group and allicin groups received corresponding doses of drugs by ig

and the rats in normal group and model group were given with the same volume of normal saline. After 4 weeks of intervention

the animals were sacrificed. The contents of total cholesterol (TG)

triglyceride (TC)

alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum and liver tissues were detected. The pathological changes of liver tissues were observed by haematoxylin-eosin(HE) staining. TNF-α and SREBP-1c protein expression levels in liver tissues were detected by immunohistochemistry. Result: As compared with the normal group

the TG and TC levels in serum and liver tissues

ALT and AST levels in liver tissues

as well as TNF-α and SREBP-1c protein expression levels in liver tissues were significantly increased in rats of model group (P < 0.05

P < 0.01). As compared with the model group

the TG and TC levels in serum and liver tissues

ALT and AST levels in liver tissues

as well as TNF-α and SREBP-1c protein expression levels in liver tissues were significantly decreased in rats of fenofibrate group

allicin low dose

middle dose and high dose groups (P < 0.05

P < 0.01) Conclusion: Allicin has lipid lowering and liver protective effect in NAFLD model rats induced by high fat diet

and the mechanism may be relevant to reducing the protein expression levels of TNF-α and SREBP-1c.

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