Objective: To prepare compound Ganning granules

optimize its extraction process and investigate its protective effect on acute hepatic injury induced by CCl4 in mice. Method: Ethanol extraction process of Schisandrae Chinensis Fructus was optimized by Taguchi design

water extraction process of the other herbs was optimized by orthogonal test.Compound Ganning granules was prepared through wet granulation process.Mice were randomly divided into six groups

included normal group

model group

bifendate group and high

middle

low dose group of compound Ganning granules.Acute hepatic injury mice model was induced by 0.1% CCl4.Levels of alanine aminotransferase (ALT)

aspartate aminotransferase (AST) in serum and malonialdehyde (MDA)

superoxide dismutase (SOD) in live tissue were detected. Result: After being optimized

transfer rates of schisandrin A and sodium danshensu were more than 90%.Compared with the normal group

activities of ALT

AST and level of MDA in model group were significantly increased and activity of SOD was inhibited.Compared with the model group

activities of ALT

AST and level of MDA in compound Ganning granules groups were significantly decreased and activity of SOD was increased. Conclusion: These optimized processes are stable and feasible.Compound Ganning granules has hepatoprotective effect on acute hepatic injury induced by CCl4 in mice by up-regulation of activity of SOD and down-regulation of activities of ALT

AST and MDA.