Objective: To select proper macroporous resin for separation and purification of total flavonol glycosides in Kemin Qidan prescription and to determine purification process parameters. Method: Taking adsorption ratio and elution rate of total flavonol glycosides and calycosin-7-O-β-D-glucoside as indexes

impacts of D101

AB-8 and S-8 macroporous resin on contents of total flavonol glycosides and calycosin-7-O-β-D-glucoside were investigated

single factor tests were adopted to select the concentration of sample liquid

sample loading quantity and the amount of 70% ethanol. Result: Optimum purification process was that condensing the sample solution to a relative density of 1.05 at 60 ℃

then centrifuging;centrifugal fluid was diluted to 0.5 g · mL-1

AB-8 type macroporous resin was washed by 1 BV of water

whose diameter and height ratio was 1: 6.The flow velocity of sample loading absorption and edulcoration was 2 BV · h-1 and sample loading quantity was that each gram resin matched with solution containing 1.5 times raw medicinal material.Eventually

eluting with 4 BV of 70% ethanol by flow velocity of 4 BV · h-1 and then collecting eluent.Transfer rates of total flavonol glycosides and calycosin-7-O-β-D-glucoside were 78.22% and 43.81%. Conclusion: This optimized purification process is credible

stable and applicable to mass production of Kemin Qidan prescription.