Objective: There is a rich resource of Tephrosia in China. This paper is to investigate the chemical components from the genius of Tephrosia purpurea. Method: The chemical compounds of T. purpurea branches and leaves were isolated and extracted

and their structures were identified by using several chromatographic separation methods

modern spectrum analysis method and literature comparison. Result: Twelve isoflavones were obtained from the branches and leaves of T. purpurea and identified as follows: 12a-dehydro-6-hydroxysumatrol (1)

elongatin (2)

tephcalostan C (3)

7

4'-dihydroxy-3'

5'-dimethoxyisoflavone (4)

4'-demethyltoxicarol isoflavone (5)

tephcalostan B (6)

5

7-di-O-prenylbiochanin A (7)

tephcalostan D (8)

tephcalostan (9)

6a

12a-dehydro-2

3

6-trimethoxy-8- (3'

3'-dimethylallyl)-9

11-dihydroxyrotenone (10)

3

4

8

9-dimethylenedioxypterocarpan (11)

12a-hydroxy-β-toxicarol (12). Conclusion: All the other 11 compounds except compound 4 were obtained from this plant for the first time.