Simultaneous Determination of Four Constituents in  by RP-HPLC

ZHAN Shi-yin

doi:10.13422/j.cnki.syfjx.2016140098

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Simultaneous Determination of Four Constituents in by RP-HPLC

更新时间：2024-01-04

- Simultaneous Determination of Four Constituents in by RP-HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 14, Pages: 98-101(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016140098
  CLC： O657.72;R284.1
- Published：2016
- 稿件说明：
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ZHAN Shi-yin. Simultaneous Determination of Four Constituents in by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(14): 98-101.
DOI：

ZHAN Shi-yin. Simultaneous Determination of Four Constituents in by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(14): 98-101. DOI： 10.13422/j.cnki.syfjx.2016140098.

摘要

目的：建立反相高效液相色谱法同时测定路边青药材中咖啡酸、槲皮素-3-O-葡萄糖苷、山柰酚-3-O-葡萄糖苷、山柰酚含量的方法。方法：采用Agilent C18色谱柱（4.6mm×250mm，5μm），流动相乙腈-0.5%甲酸水溶液梯度洗脱，流速0.8mL·min-1，柱温25℃，检测波长340nm。结果：咖啡酸、槲皮素-3-O-葡萄糖苷、山柰酚-3-O-葡萄糖苷、山柰酚分别在0.045~1.130，0.095~2.370，0.105~2.635，0.041~1.030μg线性关系良好，平均加样回收率（n=6）分别为99.0%，98.5%，97.7%，100.7%，RSD分别为1.0%，0.9%，1.0%，1.6%。结论：该方法简便、快捷，重复性好，可用于路边青药材的质量控制。

Abstract

Objective: To establish a method for simultaneously determining caffeic acid

quercetin-3-O-glucoside

kaempferol-3-O-glucoside and kaempferol in Geum aleppicum by RP-HPLC. Method: The quantitative analysis of G. aleppicum was carried out on Agilent C18 column(4.6 mm×250 mm

5 μm)

mobile phase was acetonitrile-0.5% formic acid with gradient elution

flow rate was 0.8 mL ·min-1

columntemperature was maintained at 25 ℃

and detection wavelengrt was 340 nm. Result: The linear relationships of caffeic acid

quercetin-3-O-glucoside

kaempferol-3-O-glucoside and kaempferol fell within the ranges of 0.045-1.130

0.095-2.370

0.105-2.635

0.041-1.030 μg

respectively. Their average recoveries (n=6) were 99.0%

98.5%

97.7%

100.7% with the relative standard deviations (RSDs) of 1.0%

0.9%

1.0%

1.6%

respectively. Conclusion: This method is simple

accurate

sensitive

which can be used for quality control of G. aleppicum.

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Related Author

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Related Institution

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