Simultaneous Determination of Saponins in Shenqi Compound Granules by HPLC-ELSD

SHAN Li-fang; YUAN Cai; YANG Hong-mei; CAO Lei; TAN Jing; GAO Tian; YUAN Qiang-hua; SONG Ying

doi:10.13422/j.cnki.syfjx.2016140102

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Simultaneous Determination of Saponins in Shenqi Compound Granules by HPLC-ELSD

更新时间：2024-01-04

- Simultaneous Determination of Saponins in Shenqi Compound Granules by HPLC-ELSD
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 14, Pages: 102-105(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016140102
  CLC： O657.72;R286.0
- Published：2016
- 稿件说明：
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SHAN Li-fang, YUAN Cai, YANG Hong-mei, et al. Simultaneous Determination of Saponins in Shenqi Compound Granules by HPLC-ELSD[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(14): 102-105.
DOI：

SHAN Li-fang, YUAN Cai, YANG Hong-mei, et al. Simultaneous Determination of Saponins in Shenqi Compound Granules by HPLC-ELSD[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(14): 102-105. DOI： 10.13422/j.cnki.syfjx.2016140102.

摘要

目的：建立同时测定参芪复方颗粒中人参皂苷Rg1，Re，Rb1和黄芪甲苷含量的方法，为该制剂的质量控制提供参考。方法：采用HPLC-ELSD，流动相水（A）-乙腈（B）梯度洗脱（0~3min，81%A;3~20min，81%~78%A;20~28min，78%~77%A;28~33min，77%~75%A;33~40min，75%~65.7%A;40~52min，65.7%~70%A;52~62min，70%A），流速0.8mL·min-1，柱温25℃，气体流量2.6L·min-1，漂移管温度102℃。结果:人参皂苷Rg1，Re，Rb1和黄芪甲苷分别在0.196~2.976，0.207~3.138，0.191~2.895，0.190~2.879μg与色谱峰面积呈良好线性关系，平均回收率分别为99.27%（RSD1.5%），99.33%（RSD1.1%），99.32%（RSD1.0%），99.27%（RSD2.4%）。结论:该含量测定方法同时测定参芪复方颗粒中多种指标成分时，精密度高、重复性好，可用于该制剂的质量控制。

Abstract

Objective: To develop a method for determining four kinds of saponins in Shenqi compound granules. Method: Quantitative analysis was carried out on a column of Phenomenex C18 (4.6 mm×250 mm

5 μm) by HPLC-ELSD with mobile phase of acetonitrile-water under a flow rate of 0.8 mL · min-1

column temperature was 25 ℃

flow rate of carrier gas was 2.6 L · min-1

drift tube temperature was 102 ℃. Result: Linear ranges of ginsenoside Rg1

ginsenoside Re

ginsenoside Rb1and astragaloside Ⅳ were 0.196-2.976

0.207-3.138

0.191-2.895

0.190-2.879 μg;their recoveries were 99.27%

99.33%

99.32%

99.27%

RSDs were 1.5%

1.1%

1.0% and 2.4%

respectively. Conclusion: This method is accurate

reproducible and convenient for quality control over Shenqi compound granules.

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