Objective: To establish an in vitro blood-brain barrier

in order to study the effects of Buyang Huanwu Tang on blood-brain barrier function and associated protein expression under the condition of oxygen and glucose deprivation-reperfusion injury. Method: The in vitro oxygen and glucose deprivation-reperfusion injury model was established by culturing the isolated primary rat brain microvascular endothelial cells. The cells were divided into three groups

namely blank group

model group and Buyang Huanwu Tang group

and then were processed by following steps. Cell counting kit(CCK-8) method was used to detect cell activity; Transwell assay was used to detect the permeation of bovine serum albumin(BSA); Western blot assay was used to detect the protein expressions of Occludin

ZO-1.Real-time fluorescent quantitative PCR(qPCR) was used to detect the mRNA expressions of Occludin and ZO-1. Result: Compared with the blank group

the model group showed significant decreases in cell activity

Occludin and ZO-1 protein and their mRNA expressions

and increase in BSA permeation rate(P<0.05). In Buyang Huanwu Tang group

cell survival rate was significantly increased; bovine serum albumin (BSA) transmittance reduced; Occludin

ZO-1 protein and mRNA expression increased(P<0.05). Conclusion: Buyang Huanwu Tang can reduce the oxygen glucose deprivation-reperfusion damage in the blood-brain barrier model in vitro and up-regulate ZO-1

Occludin protein and mRNA expression.