WU Cui, GAO Yue-rui, CHAO Zhi-mao, et al. Correlation Between 5-Hydroxymethylfurfural Content in Schisandra Chinensis Fructus and Its Storage and Color[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(15): 24-27.
DOI:
WU Cui, GAO Yue-rui, CHAO Zhi-mao, et al. Correlation Between 5-Hydroxymethylfurfural Content in Schisandra Chinensis Fructus and Its Storage and Color[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(15): 24-27. DOI: 10.13422/j.cnki.syfjx.2016150024.
Correlation Between 5-Hydroxymethylfurfural Content in Schisandra Chinensis Fructus and Its Storage and Color
Objective: To establish a high performance liquid chromatography (HPLC) method for content determination of 5-hydroxymethylfurfural (5-HMF) in Schisandra Chinensis Fructus (SCF)
determine the content of 5-HMF in commercial SCF and Schisandra processed with vinegar (SPV) decoction pieces
and carry out the correlative analysis between 5-HMF content and its storage and color. Method: HPLC-PDA method was performed on ODS column (4.6 mm×250 mm
5 μm) with methanol-water (5: 95) as the mobile phase at a flow rate of 1 mL·min-1. The detective wavelength was set at 284 nm and the column temperature was maintained at 30℃. Result: The HPLC method for content determination of 5-HMF in SCF and SPV was established. The contents of 5-HMF in SCF and SPV were 3.6-70.2
3 719.0-14 486.0 μg·g-1
respectively. Conclusion: The content of 5-HMF in SPV is significantly higher than that in SCF. With the extension of storage time of SCF
the color gradually deepens and the content of 5-HMF increases.
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Related Author
SUN Wen
CHAO Zhi-mao
WANG Chun
WU Xiao-yi
TAN Zhi-gao
LI Xiuqing
JI Baoyu
HE Jianglong
Related Institution
School of Pharmacy, Henan University of Traditional Chinese Medicine
Henan Provincial Herbal Ecological Planting Engineering Technology Research Center
Henan Key Laboratory of Resource and Chemistry for Chinese Traditional Medicine
Collaborative Innovation Center of Research and Development on the Whole Industry Chain of Yu-yao
Institute of Chinese Materia Medica, Storage & Packaging Position, Chinese Medicinal Material China Agriculture Research System, China Academy of Chinese Medical Sciences