Chemical Constituents in Ethanol Extract from Semiaquilegiae Radix Based on HPLC-ESI-Q-TOF-MS

YAN Qiu-ying; CHENG Hai-bo; SUN Dong-dong; TAN Jia-ni; DOU Zhi-hua

doi:10.13422/j.cnki.syfjx.2016150042

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Chemical Constituents in Ethanol Extract from Semiaquilegiae Radix Based on HPLC-ESI-Q-TOF-MS

更新时间：2024-09-23

- Chemical Constituents in Ethanol Extract from Semiaquilegiae Radix Based on HPLC-ESI-Q-TOF-MS
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 15, Pages: 42-46(2016)
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- DOI：10.13422/j.cnki.syfjx.2016150042
  CLC： R284;O657.63
- Published：2016
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YAN Qiu-ying, CHENG Hai-bo, SUN Dong-dong, et al. Chemical Constituents in Ethanol Extract from Semiaquilegiae Radix Based on HPLC-ESI-Q-TOF-MS[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(15): 42-46.
DOI：

YAN Qiu-ying, CHENG Hai-bo, SUN Dong-dong, et al. Chemical Constituents in Ethanol Extract from Semiaquilegiae Radix Based on HPLC-ESI-Q-TOF-MS[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(15): 42-46. DOI： 10.13422/j.cnki.syfjx.2016150042.

摘要

目的：采用高效液相色谱与电喷雾四极杆飞行时间质谱仪串联技术（HPLC-ESI-Q-TOF-MS）对天葵子95%乙醇提取部位中的化学成分进行定性分析。方法：采用液质联用色谱方法（LC-MS-MS），C18色谱柱（4.6 mm×150 mm，5 μm），以乙腈-0.1%甲酸溶液为流动相梯度洗脱，质谱使用ESI离子源，正离子模式采集数据。质量扫描范围为m/z 50～1 500。结果：通过质谱数据分析，并与相关文献对照，从天葵子95%乙醇提取部位共鉴定出11种化合物，分别为4-（甲氧羰基）-2-氨基丁酸、紫草氰苷、耧斗菜内酯、蝙蝠葛内酯、蝙蝠葛氰苷、菲律宾厚壳树苷、天葵氰苷、橙黄胡椒酰胺、对苯二甲酸二丁酯、胡萝卜苷及邻苯二甲酸-二-2-乙基-己酯。结论：基于HPLC-ESI-Q-TOF技术，通过高效液相色谱分离，质谱测定相对分子质量、裂解数据，文献分析检索，能够快速准确地对天葵子95%乙醇提取部位的化学成分进行分离鉴定，为进一步研究天葵子的药效物质基础提供有力证据。

Abstract

Objective: To analyze the chemical constituents of 95%ethanol extract of Semiaquilegiae Radix by using HPLC-ESI-Q-TOF-MS. Method: C18 column (4.6 mm×150 mm

5 μm) was adopted

with acetonitrile-0.1% formic acid as the mobile phase for gradient elution; ESI ion source was used as mass spectra

and the data were collected in the positive mode. The mass scanning range was between m/z 50-1 500. Result: Eleven components were identified from 95%ethanol extract of Semiaquilegiae Radix by analyzing mass spectra information

and comparing with data from relevant literatures. Conclusion: Based on HPLC-ESI-Q-TOF-MS technology

relative molecular mass and spectrometry pyrolysis data were determined

and chemical components of Semiaquilegiae Radix were determined by literature analysis and retrieval. This study provides powerful evidence for further studying the pharmacodynamics material basis of Semiaquilegiae Radix.

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