Objective: To establish an ultra-high performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-MS-MS) method for simultaneous determination of four terpene lactones in ginkgo folium extract from different manufacturers. Method: Chromatographic separation was carried out on ACQUITY UPLC BEH C18 column (2.1 mm×100 mm

1.7 μm) with water-methanol (70: 30) as the mobile phase

at a flow rate of 0.4 mL·min-1. The column temperature was set at 30℃; Waters XevoTM TQ worked in multiple reaction monitoring (MRM) mode for negative ion detection. Result: The tested constituents were separated well within 5 min

and showed good linear relationship with the peak areas within corresponding concentration range (r>0.992 3). The average recoveries ranged from 98.13%to 102.08%with RSD value below 3.0%. There was difference in contents of total terpene lactones in Ginkgo Folium extract from different manufacturers

but all the contents complied with the requirements of Pharmacopoeia of the People's Republic of China. Conclusion: This method was simple and fast with high precision

sensitivity and repeatability

which can be used for rapid quantitative analysis of terpene lactones in Ginkgo Folium extract.