Rapid Identification of Chemical Constituents in Danshen-chuanxiongqin Injection by Using UPLC-LTQ-Orbitrap Mass Spectrometer

SUN Jin-hua; LI Xian-rong

doi:10.13422/j.cnki.syfjx.2016150064

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Rapid Identification of Chemical Constituents in Danshen-chuanxiongqin Injection by Using UPLC-LTQ-Orbitrap Mass Spectrometer

更新时间：2024-09-23

- Rapid Identification of Chemical Constituents in Danshen-chuanxiongqin Injection by Using UPLC-LTQ-Orbitrap Mass Spectrometer
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 15, Pages: 64-67(2016)
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- DOI：10.13422/j.cnki.syfjx.2016150064
  CLC： R286.0
- Published：2016
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SUN Jin-hua, LI Xian-rong. Rapid Identification of Chemical Constituents in Danshen-chuanxiongqin Injection by Using UPLC-LTQ-Orbitrap Mass Spectrometer[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(15): 64-67.
DOI：

SUN Jin-hua, LI Xian-rong. Rapid Identification of Chemical Constituents in Danshen-chuanxiongqin Injection by Using UPLC-LTQ-Orbitrap Mass Spectrometer[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(15): 64-67. DOI： 10.13422/j.cnki.syfjx.2016150064.

摘要

目的：采用超高效液相结合二维线性离子阱静电轨道阱组合式高分辨质谱（UPLC-LTQ-Orbitrap）对丹参川芎嗪注射液化学成分进行快速分析和鉴别。方法：采用ACQUITY UPLC BEH C18色谱柱（2.1 mm×50 mm，1.7 μm），以乙腈-0.1%甲酸水为流动相进行梯度洗脱，流速300 μL·min-1，进样量5 μL。质谱条件为电喷雾离子源（ESI），负离子模式下检测，质量扫描范围m/z 100～1 000。结果：从丹参川芎嗪注射液中共鉴别出15个化学成分。结论： UPLC-LTQ-Orbitrap方法能快速鉴别丹参川芎嗪注射液中的化学成分，为丹参川芎嗪注射液的药效物质研究奠定基础。

Abstract

Objective: To quickly analyze and identify the chemical constituents in Danshen-chuanxiongqin injection by using UPLC-LTQ-Orbitrap mass spectrometer. Method: The separation was performed on ACQUITY UPLC BEH C18 column (2.1 mm×50 mm

1.7 μm) with acetonitrile (A) and 0.1%formic acid solution (B) as the mobile phase for gradient elution. The sample size was 5 μL. Electrospray ion source (ESI) was applied for qualitative analysis under the negative ion mode. The flow rate was 300 μL·min-1 and mass scan range was m/z 100-1 000. Result: Fifteen chemical compounds were identified from Danshen-chuanxiongqin injection. Conclusion: UPLC-LTQ-Orbitrap method be used to quickly identify the chemical components of Danshen-chuanxiongqin injection

laying a foundation for further studying the pharmacodynamics substances of Danshen-chuanxiongqin injection.

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