Fingerprint Analysis of XiaoaipingInjection and Simultaneous Determination of Its Seven Active Steroidal Components in XiaoaipingInjection by HPLC-ELSD
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Fingerprint Analysis of XiaoaipingInjection and Simultaneous Determination of Its Seven Active Steroidal Components in XiaoaipingInjection by HPLC-ELSD
Chinese Journal of Experimental Traditional Medical FormulaeVol. 22, Issue 15, Pages: 68-72(2016)
GONG Xiao-bin, ZENG Qing-hua, ZHANG Feng, et al. Fingerprint Analysis of XiaoaipingInjection and Simultaneous Determination of Its Seven Active Steroidal Components in XiaoaipingInjection by HPLC-ELSD[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(15): 68-72.
DOI:
GONG Xiao-bin, ZENG Qing-hua, ZHANG Feng, et al. Fingerprint Analysis of XiaoaipingInjection and Simultaneous Determination of Its Seven Active Steroidal Components in XiaoaipingInjection by HPLC-ELSD[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(15): 68-72. DOI: 10.13422/j.cnki.syfjx.2016150068.
Fingerprint Analysis of XiaoaipingInjection and Simultaneous Determination of Its Seven Active Steroidal Components in XiaoaipingInjection by HPLC-ELSD
Objective: To develop and validate a high performance liquid chromatography coupled with evaporative light scattering detector (HPLC-ELSD) method for the quantitative determination and fingerprint analysis of 7 active steroidal components in Xiaoaiping injection. Method: Chromatographic separation was performed on an Eclipse XDB-C18column (4.6 mm×250 mm
5 μm) by gradient elution using acetic acid in acetonitrile solution at a flow rate of 0.8 mL·min-1. The drift tube temperature of ELSD was set to 60℃ and nebulizer gas pressure was 0.4 MPa. Method validation was performed to assure its linearity
limits of detection and quantification
precision
repeatability
stability and accuracy. Result: Fingerprint analysis obtained 13 common peaks in Xiaoaiping injection
and the similarity was higher than 0.94. The HPLC-ELSD method allowed the quantification of 7 compounds (17β-tenacigenin B
tenacigenin B
tenacigenin A
tenacigenoside A
marsdenoside I and tenacissoside F)
and was successfully applied to fingerprint analysis for 11 batches of Xiaoaiping injection. Conclusion: It was the first time to apply HPLC-ELSD for simultaneous determination of 7 active ingredients and fingerprint analysis of Xiaoaiping injection. The results proved the reliability and comprehensiveness of the combination of quantitative analysis for marker ingredients and chemical fingerprint for TCM formula.