LI Jun-mao, HE Ming-zhen, LI Tian-er, et al. Fragmentation Behavior of Isorhynchophylline in ESI-MS and Its Application in Identification of Metabolites[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(15): 78-84.
DOI:
LI Jun-mao, HE Ming-zhen, LI Tian-er, et al. Fragmentation Behavior of Isorhynchophylline in ESI-MS and Its Application in Identification of Metabolites[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(15): 78-84. DOI: 10.13422/j.cnki.syfjx.2016150078.
Fragmentation Behavior of Isorhynchophylline in ESI-MS and Its Application in Identification of Metabolites
Objective: To analyze in vivo metabolic pathways and products of isorhynchophylline in rats. Method: UPLC-Q-TOF-MS with positive ion mode was established and applied to identify the metabolites of isorhynchophylline in rat plasma after intragastric administration of 37.5 mg·kg-1
mobile phase was 0.1%formic acid and acetonitrile for gradient elution. Result: A total of 13 compounds
including 1 prototype and 12 metabolites (11 of phase Ⅰ and 1 of phaseⅡ) were identified by compared with the blank biological samples and the fragmentation mechanism of MS spectra of reference substances. Conclusion: The metabolic pathway of isorhynchophylline in rats is summarized as oxidation
stereoisomerism
hydroxylation
dehydrogenation
hydrolyzation and glucuronidation.This paper enriches ESI-MS fragmentation rules of indole alkaloids and provides reference for further mass spectrometry analysis of such compounds.
Distribution of Isorhynchophyline in Liver Tissures before and after Compatibility with Gastrodin and Isorhynchophylline in Rats
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Related Author
CHEN Zhi-yuan
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ZHANG Sitong
HU Xianrun
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Related Institution
Key Laboratory for Standardization of Chinese Medicines,Ministry of Education, Shanghai Key Laboratory for Compound Chinese Medicines,Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine
Key Laboratory of Modern Preparation of Traditional Chinese Medicine of Ministry of Education, Jiangxi University of Chinese Medicine
State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, The First Affiliated Hospital of Xinjiang Medical University
Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine