Objective: To observe the protection effect of extract from Xinjiang mulberry on carbon tetrachloride (CCl4) and alcohol-induced acute liver injury in mice. Method: Totally 60 Kunming mice (30 males and 30 females) were randomly divided into 6 groups:normal group and model group with intragastric administration of distilled water

positive group (bifendate pills

0.40 g·kg-1)

Xinjiang mulberry extract low dose group

middle dose group and high dose group (1.525

3.050

6.100 g·kg-1). All the other mice except those in normal group received CCl4 and alcohol to establish acute liver injury models in mice. After successful modeling

the mice in all groups received intragastric administration for continuous 7 days. The liver coefficient in mice was determined after drug administration

and colorimetric method was used to determine the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum of CCl4 liver injury mice

and the contents of triglyceride (TG)

malondiadehyde (MDA) and glutathione (GSH) in liver homogenate of alcoholic liver injury mice. HE staining method was used to observe the pathological section of liver tissues in mice. Result: As compared with the normal group

the activities of ALT and AST in serum of CCl4 liver injury mice in model group were significantly increased (P<0.01);the contents of MDA and TG in liver tissues of alcoholic liver injury mice in model group were significantly increased while the content of GSH was significantly reduced (P<0.05

P<0.01);the liver injury was more obvious in the model group during the pathological observation. As compared with the model group

the extract from Xinjiang mulberry could reduce the activities of ALT and AST in serum

significantly reduce the contents of TG and MDA

and significantly increase the content of GSH

with statistically significant differences (P<0.05

P<0.01). The pathological examination results indicated that the extract from Xinjiang mulberry had obvious liver-protecting effect. Conclusion: The extract from Xinjiang mulberry has notable protective effect for the CCl4 and alcohol-induced liver injury in mice.