ZHAO Xin, ZHANG Xue-lan, JIANG Qiu, et al. Comparison of Gastrointestinal Absorption of Oleuropein and Hydroxytyrosol in Rats[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(16): 6-9.
DOI:
ZHAO Xin, ZHANG Xue-lan, JIANG Qiu, et al. Comparison of Gastrointestinal Absorption of Oleuropein and Hydroxytyrosol in Rats[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(16): 6-9. DOI: 10.13422/j.cnki.syfjx.2016160006.
Comparison of Gastrointestinal Absorption of Oleuropein and Hydroxytyrosol in Rats
Objective: To compare difference between gastrointestinal absorption of oleuropein and its aglycone (hydroxytyrosol) in rats
analyze metabolites of oleuropein in rats
and provide a scientific basis for research on synergistic mechanism of Ligustri Lucidi Fructus steamed with wine. Method: Gastrointestinal perfusion model of rats was used
contents of oleuropein and hydroxytyrosol in the gastrointestinal perfusion effluent of rats were detected by HPLC after administrating at different time points
absorption rate constant (Ka) and cumulative absorption rate were calculated.The mobile phase was acetonitrile and 0.1%formic acid aqueous solution for gradient elution
detection wavelength was set at 240 nm.By positioning of oleuropein and hydroxytyrosol reference
metabolites of oleuropein in rat's stomach and small intestine were determined. Result: Cumulative absorption rate in 4 h and Ka of hydroxytyrosol in rat stomach and small intestine were significantly higher than oleuropein.Hydroxytyrosol can be detected in rat stomach and intestine metabolites after pouring into oleuropein. Conclusion: Compared with oleuropein
hydroxytyrosol is more conducive to be absorbed by the body.Oleuropein can be metabolized into hydroxytyrosol both in rat stomach and small intestine.
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Related Author
HUO Yu-jia
YUE Lin
LIU Ying
SU Hui
FU Ya-dong
LI Li
WANG Cheng-yong
ZHANG Jianwei
Related Institution
Third Grade Laboratory of Traditional Chinese Medicine(TCM) Preparations,National Administration of TCM,Shuguang Hospital Affiliated to Shanghai University of TCM
Shandong Academy of Chinese Medicine
Occupational Diseases Hospital of Shandong First Medical University
The Affiliated Hospital of Guizhou Medical University
Center for Drug Inspection,Guizhou Medical Products Administration