PANG Xiao-chen, LIU Zhi-dong, HUANG Rui, et al. Optimization of Extraction Technology of Gangxi Ⅱ Gels by Multi-index Weighted Scoring Method[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(16): 22-24.
DOI:
PANG Xiao-chen, LIU Zhi-dong, HUANG Rui, et al. Optimization of Extraction Technology of Gangxi Ⅱ Gels by Multi-index Weighted Scoring Method[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(16): 22-24. DOI: 10.13422/j.cnki.syfjx.2016160022.
Optimization of Extraction Technology of Gangxi Ⅱ Gels by Multi-index Weighted Scoring Method
Objective: To optimize extraction conditions of Gangxi Ⅱ gels. Method: UPLC was employed to determine contents of berberine hydrochloride
rutin and quercetin in gels with mobile phase of acetonitrile-0.1%formic acid solution for gradient elution
flow rate of 0.3 mL·min-1 and detection wavelength at 260 nm.Taking composite score of extracting amounts of berberine hydrochloride
rutin and quercetin as index
orthogonal test and single factor tests were adopted to optimize extraction process. Result: Optimum extraction technology was:extracted thrice with 10 times the amount of water for 1 hour of per time;under these conditions
extracting amounts of rutin
berberine hydrochloride and quercetin were 9.154
1.264
0.734 mg·g-1
respectively. Conclusion: This optimized technology is stable and feasible
which offers expermental basis for further development of Gangxi Ⅱ gels.