LIU Rui, ZHANG Hong-chi, LI Hui, et al. Isolation of Secondary Metabolites of Endophytic Fungus from var. and Identification of Strain[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(16): 25-29.
DOI:
LIU Rui, ZHANG Hong-chi, LI Hui, et al. Isolation of Secondary Metabolites of Endophytic Fungus from var. and Identification of Strain[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(16): 25-29. DOI: 10.13422/j.cnki.syfjx.2016160025.
Isolation of Secondary Metabolites of Endophytic Fungus from var. and Identification of Strain
Objective: To study the secondary metabolites from endophytic fungus of Astragalus membranaceus. Method: The compounds were isolated by repeated silica gel column chromatography combined with Sephadex LH-20 gel chromatography and identified on the basis of physic-chemical constants and spectral analysis. Result: Eight compounds were obtained and elucidated as 23-methylergosta-7
22-diene-3β
5α
6α-triol (1)
5α
8α-epidioxy-ergosta-6
9(11)
22-trien-3β-ol (2)
cyclotryprostatins A (3)
cyclotryprostatins C (4)
trypacidin (5)
monomethylsulochrin (6)
fumiquinazoline D (7) and fumiquinazoline J (8). Conclusion: The eight compounds were obtained from the endophytic fungus of Astragalus membranaceus for the first time.
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