Simultaneous Determination of Three Components in Benzoinum by HPLC

LIU Yao; LI Bi-jun; WANG Feng

doi:10.13422/j.cnki.syfjx.2016160037

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Simultaneous Determination of Three Components in Benzoinum by HPLC

更新时间：2024-01-04

- Simultaneous Determination of Three Components in Benzoinum by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 16, Pages: 40-43(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016160037
  CLC： O657.72;R284.1
- Published：2016
- 稿件说明：
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LIU Yao, LI Bi-jun, WANG Feng. Simultaneous Determination of Three Components in Benzoinum by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(16): 40-43.
DOI：

LIU Yao, LI Bi-jun, WANG Feng. Simultaneous Determination of Three Components in Benzoinum by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(16): 40-43. DOI： 10.13422/j.cnki.syfjx.2016160037.

摘要

目的：建立HPLC同时测定安息香中香草醛、松柏醛和trans-[四氢-2-（4-羟基-3-甲氧基苯基）-5-氧代呋喃-3-基]甲基苯甲酸酯含量的方法。方法：采用Phenomenex Luna C18（2）色谱柱（4.6 mm×250 mm，5μm），以甲醇-水为流动相，在1.0 mL·min-1的流速下，对样品进行梯度洗脱，并在280 nm波长处测定这3种化学成分的含量。结果： 3种成分均能达到基线分离，且线性关系良好，加样回收率分别为99.51%，101.25%，100.71%。结论：该方法灵敏、准确、具有良好的稳定性和重复性，可用于同时测定安息香中这3种化学成分的含量，为安息香的质量控制研究提供一定参考。

Abstract

Objective: To simultaneously determine the content of three components vanillin

coniferyl aldehyde

trans-[tetrahydro-2-(4-hydroxy-3-methoxyphenyl)-5-oxofuran-3-yl] methyl benzoate in the Benzoinum by HPLC. Method: HPLC was performed on a Phenomenex Luna C18(2) (4.6 mm×250 mm

5 μm) column

with methanol and water as the mobile phase for gradient elution. The flow rate was 1 mL·min-1. The detection wavelength was 280 nm. Result: All of the three components achieved baseline separation

with a good linear relationship. The average recovery rate was 99.51%

101.25%

100.71%respectively. Conclusion: This method is sensitive

accurate

reproducible and stable

and so can be used for the determination of the three components in Benzoinum

and provide reference for the quality control over the Benzoinum.

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Related Institution

Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine，Hainan Branch of Institute of Medicinal Plant Development， Chinese Academy of Medical Sciences & Peking Union Medical College

School of Traditional Chinese Medicine(TCM), Guangdong Pharmaceutical University

Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica of State Administration of TCM

Engineering & Technology Research Center for Chinese Materia Medica Quality of the Universities of Guangdong Province

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