ZENG Ting, PENG Mei, YANG Juan. Isolation, Purification and Structural Analysis of Curculiginis Rhizoma Polysaccharide[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(16): 53-57.
DOI:
ZENG Ting, PENG Mei, YANG Juan. Isolation, Purification and Structural Analysis of Curculiginis Rhizoma Polysaccharide[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(16): 53-57. DOI: 10.13422/j.cnki.syfjx.2016160053.
Isolation, Purification and Structural Analysis of Curculiginis Rhizoma Polysaccharide
puriffication and structural analysis of the polysaccharides from the Curculiginis Rhizoma. The work will provide the theoretical foundation for the further application of Curculiginis Rhizoma polysaccharides. Method: The crude polysaccharide was obtained by hot water extraction
ethanol precipitation and freezedrying. After further purification by DEAE-cellulose and Sepharose CL-6B column chromatography
pure polysaccharide (XMP-1) was achieved. The structural of XMP-1 was elucidated by GPC
UV
IR and GC-MS. Result: XMP-1 was composed of mannose
glucose
galactose with a molar ratio of 81.2:5.7:1. Total carbohydrate content of XMP-1 was 91.8% and the molecular weight was 3 031 Da. XMP-1 have no nucleic aicd
protein and pigment. IR chromatography showed that XMP-1 has pyrane characteristic absorption peak. XMP-1 in molecular conformation in aqueous solution can be naturally curly structure. Conclusion: XMP-1 is a kind of small molecular weight neutral pure heterosaccharides.
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