Objective: To study the changes in chemical component content in fungal substance of Trametes robiniophila/Isatidis Radix co-fermentation system. Method: Chloroform and water were used respectively to extract fat-soluble and water-soluble components in fungal substance at the different fermentation time. The fat-soluble and water-soluble fractions of the co-fermentation system were analyzed by UPLC-MS

and the obviously changed chromatographic peak in the fermentation process was identified to determine the molecular structure. Result: There were 11 chromatographic peaks isolated in the fat-soluble part of Trametes robiniophila/Isatidis Radix fungal substance

and eight compounds were identified by mass spectrometry. They were 3-formaldehyde indole

hypoxanthine

2

4-quinazoline diketone

clove acid

(E)-3-(3'

5'-2) methoxy-4'-(hydroxy benzal)-2-indole ketone

2

5-2 hydroxy indole

guanine

and indigo red

respectively. In the water-soluble part

eight chromatographic peaks were isolated and identified by mass spectrometry. The five compounds were arginine

licorice essence

adenine

epigoitrin

and lubricious amine ketone

respectively. Conclusion: The 13 isolated compounds with various pharmacodynamics effects provide a theoretical basis for follow-up pharmacodynamics studies.