Protective Effects of Campsis Flos Total Flavonoids (CFTF) on Cerebral Ischemia Reperfusion Injury in Rats

FANG Xiao-yan; WU Su-hui; WANG Lin-lin; ZHENG Yan; MIAO Ming-san

doi:10.13422/j.cnki.syfjx.2016160109

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Protective Effects of Campsis Flos Total Flavonoids (CFTF) on Cerebral Ischemia Reperfusion Injury in Rats

更新时间：2024-01-04

- Protective Effects of Campsis Flos Total Flavonoids (CFTF) on Cerebral Ischemia Reperfusion Injury in Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 16, Pages: 109-113(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016160109
  CLC： R285.5
- Published：2016
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FANG Xiao-yan, WU Su-hui, WANG Lin-lin, et al. Protective Effects of Campsis Flos Total Flavonoids (CFTF) on Cerebral Ischemia Reperfusion Injury in Rats[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(16): 109-113.
DOI：

FANG Xiao-yan, WU Su-hui, WANG Lin-lin, et al. Protective Effects of Campsis Flos Total Flavonoids (CFTF) on Cerebral Ischemia Reperfusion Injury in Rats[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(16): 109-113. DOI： 10.13422/j.cnki.syfjx.2016160109.

摘要

目的：研究凌霄花总黄酮对脑缺血再灌注损伤大鼠的保护作用，并探讨其作用机制。方法： SPF级Wistar大鼠60只，随机分为假手术组，模型组，阳性药组（阿司匹林片，50 mg·kg-1），凌霄花总黄酮高、低剂量组（200，100 mg·kg-1），除假手术组外，建立短暂性大脑中动脉阻塞大鼠模型（tMCAO），采用神经缺损评分（NDS）法评价行为学的改变、苏木素-伊红（HE）染色法评价脑组织病理形态的改变、酶联免疫吸附测定（ELISA）检测血清中神经元特异性烯醇化酶（NSE）的水平及脑组织中细胞因子白细胞介素-10（IL-10），转化生长因子-β1（TGF-β1）的水平，探讨凌霄花总黄酮对脑缺血再灌注损伤的保护作用。结果：与假手术组比较，模型组大鼠神经缺损评分明显升高（P<0.01），脑组织病理损伤严重，血清NSE水平明显升高（P<0.01），脑组织中IL-10，TGF-β1水平明显降低（P<0.01）；与模型组比较，凌霄花总黄酮高剂量组可明显改善模型大鼠的神经缺损症状，降低神经缺损评分（P<0.05）；凌霄花总黄酮高、低剂量组和阿司匹林组能明显减轻脑组织病理损伤程度（P<0.01，P<0.05），降低血清中NSE水平（P<0.05），升高脑组织中IL-10，TGF-β1水平（P<0.01，P<0.05）。结论：凌霄花总黄酮对短暂性脑缺血再灌注大鼠有一定的保护作用，可能与降低NSE水平，升高抗炎细胞因子IL-10，TGF-β1的水平，减轻炎症损伤有关。

Abstract

Objective: To observe the protective effects of total flavonoids from Campsis Flos (CFTF) on cerebral ischemia reperfusion injury in rats. Method: Sixty SPF grade Wistar rats were randomly divided into sham operation group

model group

positive drug group (aspirin

50 mg·kg-1)

CFTF high dose and low dose groups (200

100 mg·kg-1). Models of transient middle cerebral artery occlusion (tMCAO) were established in all the rats except those in sham operation group. The behavioral injury was detected by neurologic deficit scores (NDS); the cerebral histopathology was detected by HE staining; and the levels of neuron specific enolase (NSE) in serum

interleukin(IL)-10

and TGF-β1 in brain homogenate were detected by ELISA

to discuss the protective effect of CFTF on cerebral ischemia reperfusion injury. Result: As compared with sham operation group

the neurologic deficit scores were significantly higher in rats of model group (P<0.01)

with serious cerebral histopathology injuries; and the level of NSE in serum was significantly increased (P<0.01); the levels of IL-10

and TGF-β1 in brain homogenate were decreased dramatically (P<0.01). As compared with the model group

the neurologic deficit symptoms were significantly improved in CFTF high dose group (200 mg·kg-1)

with decreased neurologic deficit scores (P<0.05); cerebral histopathology injury was significantly decreased(P<0.01

P<0.05)

NSE level in serum was decreased (P<0.05)

and levels of IL-10

TGF-β1 in brain tissues were increased (P<0.01

P<0.05) in CFTF high dose and low dose groups (200

100 mg·kg-1) and aspirin group. Conclusion: CFTF had the protective effects on tMCAO rats by decreasing the level of NSE

increasing the levels of anti-inflammation cytokines (IL-10 and TGF-β1)

and attenuating the inflammation injury.

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