Effect of Alternol on Glycolysisin in Mice Melanoma B16F0 Cells

WANG Peng-long; MA Xiao-yi; ZHENG Qiu-sheng

doi:10.13422/j.cnki.syfjx.2016160119

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Effect of Alternol on Glycolysisin in Mice Melanoma B16F0 Cells

更新时间：2024-01-04

- Effect of Alternol on Glycolysisin in Mice Melanoma B16F0 Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 16, Pages: 119-122(2016)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2016160119
  CLC： R285
- Published：2016
- 稿件说明：
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WANG Peng-long, MA Xiao-yi, ZHENG Qiu-sheng. Effect of Alternol on Glycolysisin in Mice Melanoma B16F0 Cells[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(16): 119-122.
DOI：

WANG Peng-long, MA Xiao-yi, ZHENG Qiu-sheng. Effect of Alternol on Glycolysisin in Mice Melanoma B16F0 Cells[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(16): 119-122. DOI： 10.13422/j.cnki.syfjx.2016160119.

摘要

目的：研究维泰醇对小鼠黑色素瘤B16F0细胞糖酵解水平的影响并初步探究其机制。方法：以小鼠黑色素瘤B16F0细胞为研究对象，不同浓度维泰醇处理小鼠黑色素瘤B16F0细胞，另设空白组，采用磺酰罗丹明（SRB）法检测维泰醇对细胞增殖的影响，反向高效液相色谱法检测维泰醇对B16F0细胞ATP含量影响，试剂盒检测B16F0细胞培养液中乳酸含量影响，放射同位素法测定维泰醇对B16F0细胞葡萄糖摄取影响，试剂盒检测维泰醇对B16F0细胞己糖激酶（HK），丙酮酸激酶（PK），乳酸脱氢酶（LDH）活性影响。结果：与空白组比较，维泰醇可浓度依赖性的抑制B16F0细胞ATP的产生和乳酸的释放，减少B16F0细胞对葡萄糖的摄取，浓度依赖性的降低PK和LDH的活性（P<0.05，P<0.01），但对HK的活性无显著性影响。结论：维泰醇可抑制B16F0细胞糖酵解水平，其机制可能与抑制葡萄糖摄取，下调糖酵解的关键酶PK和LDH的活性有关。

Abstract

Objective: To evaluate the effects of alternol on the level of glycolysis in melanoma B16F0 cells of mice

and to explore its mechanism. Method: Mice melanoma B16F0 cells were cultured and treated with different concentrations of alternol in vitro. The proliferation of melanoma B16F0 cells was detected by a sulforhodamine B assay. The content of adenosine triphosphate (ATP) was detected by a reverse high performance liquid chromatography method

and the content of lactic acid in the medium was detected by a lactic acid assay kit. Glucose intake of B16F0 cell was determined by radioactive isotopes. The activities of hexokinase (HK)

pyruvate kinase (PK) and lactic dehydrogenase (LDH) were detected by the related assay kits. Result: Compared with the control group

alternol could inhibit the release of lactic acid and the production of ATP

reduce glucose uptake and the activities of PK and LDH in B16F0 cells(P<0.05

P<0.01). Conclusion: alternol inhibits glycolysis in B16F0 cells

which may be related to reducing the glucose uptake and the activities of PK and LDH.

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