Analyze Difference of Chemical Compositions in  from Different Origins by UPLC-Q-TOF-MS

DENG Hao-yue; WU Yuan-yuan; YUAN Lei; WANG Yuan; TIAN Meng; LI Yu-bo; YANG Bin

doi:10.13422/j.cnki.syfjx.2016170037

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Analyze Difference of Chemical Compositions in from Different Origins by UPLC-Q-TOF-MS

更新时间：2024-01-04

- Analyze Difference of Chemical Compositions in from Different Origins by UPLC-Q-TOF-MS
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 17, Pages: 37-42(2016)
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- DOI：10.13422/j.cnki.syfjx.2016170037
  CLC： O657.63;R284.1
- Published：2016
- 稿件说明：
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DENG Hao-yue, WU Yuan-yuan, YUAN Lei, et al. Analyze Difference of Chemical Compositions in from Different Origins by UPLC-Q-TOF-MS[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(17): 37-42.
DOI：

DENG Hao-yue, WU Yuan-yuan, YUAN Lei, et al. Analyze Difference of Chemical Compositions in from Different Origins by UPLC-Q-TOF-MS[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(17): 37-42. DOI： 10.13422/j.cnki.syfjx.2016170037.

摘要

目的：应用超高效液相色谱-四级杆飞行时间质谱联用技术（UPLC-Q-TOF-MS）分析不同产地抱茎苦荬菜中化学成分差异。方法：采用Waters Acquity BEH C18色谱柱（2.1 mm×100 mm，1.7 μm），乙腈-0.1%甲酸水为流动相进行梯度洗脱，电喷雾离子源（ESI）正、负离子模式下扫描采集数据，通过准分子离子及二级碎片离子对化学成分进行鉴定。应用主成分分析（PCA）和偏最小二乘判别分析（PLS-DA）对数据进行多元统计分析。结果：从抱茎苦荬菜中鉴定出18种化学成分。其中，11，13α-dihydroixerin Z，木犀草素，3，4-二羟基苯甲酸，芹菜素-7-O-葡萄糖醛酸苷，芦丁，菊苣酸，木犀草素-7-O-葡萄糖醛酸苷及鸟嘌呤核苷为不同产地抱茎苦荬菜的主要差异成分。结论：通过该研究建立的UPLC-Q-TOF-MS多元统计分析方法，明确了不同产地抱茎苦荬菜的差异化学成分，可以为抱茎苦荬菜的质量控制提供依据。

Abstract

Objective: To study the difference of chemical components in Ixeris sonchifolia (ISH) from different origins by UPLC-Q-TOF-MS. Method: The separation was performed on a Waters Acquity BEH C18 column (2.1 mm×100 mm

1.7μm) with acetonitrile-0.1%formic solution as the mobile phase for gradient elution. Mass spectrometric data were obtained in electrospray ionization (ESI) positive and negative modes. Meanwhile

the chemical components were identified with quasi-molecular ions and secondary fragment ions. Principal component analysis (PCA) and partial least squares discriminate analysis (PLS-DA) were used for multivariate statistical analysis. Result: Eighteen chemical components were identified from ISH. Among them

13α-dihydroixerin Z

luteolin

4-dihydroxybenzoic acid

apigenin-7-O-glucuronide

rutin

chicory acid

luteolin-7-O-glucuronide and guanosine were the main differential compositions of ISH from different origins. Conclusion: The difference chemical constituents in ISH from different origins were identified by the established UPLC-Q-TOF-MS-MS method

which could provide the basis for the quality control of ISH.

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