WANG Xiu-fen, LENG Xiao-hong, CHEN Hai-yan, et al. HPLC Fingerprint of Total Alkaloids from Different Parts of [J]. Chinese journal of experimental traditional medical formulae, 2016, 22(17): 49-54.
DOI:
WANG Xiu-fen, LENG Xiao-hong, CHEN Hai-yan, et al. HPLC Fingerprint of Total Alkaloids from Different Parts of [J]. Chinese journal of experimental traditional medical formulae, 2016, 22(17): 49-54. DOI: 10.13422/j.cnki.syfjx.2016170049.
HPLC Fingerprint of Total Alkaloids from Different Parts of
Objective: To study the HPLC fingerprint of the total alkaloids from different parts of the Sophora alopecuroides
and provide the basis for scientific evaluation and effective control of the quality of total alkaloids in S. alopecuroides. Method: Totally 16 batches of S. alopecuroides from different producing areas and the total alkaloids were used to gain fingerprints by HPLC-DAD. The obtained data was analyzed by similarity evaluation
and the pattern recognition was carried out by cluster analysis and principal component analysis. Result: Fingerprints of S. alopecuroides fructus and herba S. alopecuroides extracts were established; common peaks were determined
and the chromatographic peaks were identified. Through cluster analysis and principal component analysis
the total alkaloids extract of the 16 batches Chinese medicinal materials were divided into 3 groups according to different producing areas. Conclusion: S. alopecuroides fructus and herba S. alopecuroides extracts had good fingerprint features and specificity
which could be used for the overall quality control of the total alkaloids from S. alopecuroides fructus and herba S. alopecuroides.
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Related Author
Yan MENG
Wu-yi LI
Jia-ming SHAN
Hai-fang CHEN
Xiao-ying HUANG
Ming YANG
Wu-liang YANG
Xiao-ling YU
Related Institution
Key Laboratory of Modern Preparation of Traditional Chinese Medicine (TCM),Ministry of Education,Jiangxi University of TCM
Kunming Municipal Hospital of Traditional Chinese Medicine
Yunnan Provincial Key Laboratory of Research on External Drug Delivery System and Preparation Technology in University,Yunnan University of Chinese Medicine
College of Pharmacy and Chemistry, Key Laboratory of Universities in Yunnan Province on Development of Genuine Medicinal Materials Resources in Western Yunnan, Dali University