Objective: To develop an HPLC method for the simultaneous determination of prim-O-glucosylcimifugin

ferulic acid

naringin

menthone

osthole

and isoimperatorin in Jingfang granules. Method: The chromatographic separation was achieved on a ZORBAX SB-C18(4.6 mm×250 mm

5μm) column with acetonitrile-water as mobile phases for gradient elution

at the flow rate of 1.0 mL · min-1

and the column temperature was 30℃. Result: The results showed that the six active components were well separated and showed good linear relationship within the following range

prim-O-glucosylcimifugin (5.5-27.4 mg · L-1)

ferulic acid (6.5-32.3 mg · L-1)

naringin (60.6-303.0 mg · L-1)

menthone (0.48-2.4 mg · L-1)

osthole (0.98-4.9 mg · L-1)

and isoimperatorin (3.5-17.5 mg · L-1). The average recovery was between 97.5%-101.3%. Conclusion: This method is accurate

sensitive

credible and repeatable. It can be applied for the quality control of Jingfang granules.