Determination of Four Components in Qinggan Shuyu Granule by HPLC

JIANG Hai; ZHANG Yan-yan; MENG Yong-hai; YANG Liu; YANG Bing-you; WANG Qiu-hong; KUANG Hai-xue

doi:10.13422/j.cnki.syfjx.2016170063

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Determination of Four Components in Qinggan Shuyu Granule by HPLC

更新时间：2024-01-04

- Determination of Four Components in Qinggan Shuyu Granule by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 17, Pages: 63-66(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016170063
  CLC： R286.0
- Published：2016
- 稿件说明：
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JIANG Hai, ZHANG Yan-yan, MENG Yong-hai, et al. Determination of Four Components in Qinggan Shuyu Granule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(17): 63-66.
DOI：

JIANG Hai, ZHANG Yan-yan, MENG Yong-hai, et al. Determination of Four Components in Qinggan Shuyu Granule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(17): 63-66. DOI： 10.13422/j.cnki.syfjx.2016170063.

摘要

目的：建立清肝疏郁颗粒中胡黄连苷Ⅰ，Ⅱ，芍药内酯苷和芍药苷的HPLC定量分析方法。方法：超声提取目标物；采用HPLC-DAD系统，Diamonsil（2）-C18色谱柱（4.6 mm×250 mm，5 μm），流动相乙腈-0.05%甲酸水溶液梯度洗脱，检测波长264 nm（胡黄连苷Ⅰ，Ⅱ），230 nm（芍药苷，芍药内酯苷）。结果：胡黄连苷Ⅰ，Ⅱ，芍药苷，芍药内酯苷的线性范围分别为0.067 2～6.72 μg（r=0.999 6），0.017 4～17.4 μg（r=0.999 8），0.01～10.00 μg（r=0.999 5），0.06～6.00 μg（r=0.999 1）。加样回收率分别为98.55%，100.95%，97.90%，97.90%；RSD分别为1.7%，2.4%，1.4%，1.7%。结论：该方法准确、灵敏、简便、重复性好，为清肝疏郁颗粒剂的质量控制提供了科学基础。

Abstract

Objective: To establish a rapid HPLC-DAD method for the simultaneous determination of four compounds

namely picroside Ⅰ

Ⅱ

paeoniflorin and albiflorin std in Qinggan Shuyu granule. Method: The analytes were extracted by ultrasonic. The separation of the compounds was performed on Diamonsil (2)-C18 (4.6 mm×250 mm

5μm)column with acetonitrile-0.05%formic acid solution as mobile phase at the wavelength of 264 nm (picroside Ⅰ

Ⅱ) and 230 nm (paeoniflorin

albiflorinstd). Result: The four analytes demonstrated a good linearity (r>0.9991) within a relatively wide concentration range (0.0672-6.72μg for picroside Ⅰ

0.0174-17.4μg for picroside Ⅱ

0.01-10.00μg for paeoniflorin and 0.06-6.00μg for albiflorin std)

and the recoveries ranged between 97.90%-100.95%

with RSD of 1.7%

2.4%

1.4%

1.7%

respectively. Conclusion: The method was found to be accurate

simple and sensitive for the quantitative analysis on the four compounds in Qinggan Shuyu granule.

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