MENG Xin, SHAN Jin-jun, XIE Tong, et al. Mechanism Analysis of Jinxin Oral Liquid Against Respiratory Syncytial Virus Pneumonia Based on Plasma Metabonomics[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(17): 71-76.
DOI:
MENG Xin, SHAN Jin-jun, XIE Tong, et al. Mechanism Analysis of Jinxin Oral Liquid Against Respiratory Syncytial Virus Pneumonia Based on Plasma Metabonomics[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(17): 71-76. DOI: 10.13422/j.cnki.syfjx.2016170071.
Mechanism Analysis of Jinxin Oral Liquid Against Respiratory Syncytial Virus Pneumonia Based on Plasma Metabonomics
Objective: To detect the relative content changes of endogenous metabolites in plasma of BALB/c mice by GC-MS technology
and to explore the mechanism of Jinxin oral liquid against respiratory syncytial virus (RSV) pneumonia in vivo. Method: BALB/c mice were challenged intranasally with RSV to establish the RSV pneumonia model
then intragastric administration of Jinxin oral liquid with dose of 27.6 g · kg-1 · d-1.After 3 days of inoculation
five mice in each group were hanged and inferior lobe of right lung were collected to take histopathological examination.After continuously given Jinxin oral liquid for 7 days
plasma were collected to detect the endogenous metabolites by GC-MS (m/z 50-500) and possible metabolic pathways concerned were analyzed. Result: Thirty-two endogenous metabolites in plasma were detected
arachidonic acid
glutamine
lactic acid
D-glucose
urea and 1
5-anhydro-D-sorbitol were differential metabolites among them.Metabolic pathways of archidonic acid
glutamine and pyruvate were the most concerned. Conclusion: The antiviral effect of Jinxin oral liquid against RSV pneumonia may be related to regulation of metabolic disorder caused by RSV.
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