LI Xiao-lin, ZHAN Xiao-ri, LI Ying, et al. Biological Characteristics of Seed[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(18): 27-30.
DOI:
LI Xiao-lin, ZHAN Xiao-ri, LI Ying, et al. Biological Characteristics of Seed[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(18): 27-30. DOI: 10.13422/j.cnki.syfjx.2016180027.
Objective: To provide technical reference for standardization planting and long-term storage of Salvia miltiorrhiza seed by studying seed structure
germination and storage characteristics. Method: In the study of morphology and structure
seeds and their paraffin sections were observed
and photos were taken by the dissecting microscope and electron microscope. In the study of germination
the optimal temperature and different light conditions were investigated. In the study of storage characteristics
the changes in seed viability at different water content and temperature conditions were investigated to determine optimal storage temperature and water content. Result: ① S. miltiorrhiza seeds can well germinate at 15-35℃
with the optimal temperature between 20-30℃. Fluctuating temperatures at 15/25℃ and 20/30℃ can increase the germination rate. Light can decrease the germination rate at high temperature. ② Low-temperature storage was better than room-temperature storage. Higher or lower water content could lead to the sharp decrease of seed viability. The optimal storage condition was 4℃
with 5%of water content. ③ The pericarp of S. miltiorrhiza consisted of the mucilage cell layer
the parenchyma cell layer
the single sclereid layer and the sclerenchyma cell layer. After absorbing water
the mucilage cells swelled
ruptured and released a lot of gelatinous mucus. Conclusion: S. miltiorrhiza has a wide seed germination temperature range
and can be planted in most areas of China. In seedling
fluctuating temperatures can be used to promote seed germination and increase the seedling emergence rate of S. miltiorrhiza. Temperature and water content are important factors in the storage of S. miltiorrhiza seeds. The optimal storage method is low temperature and low water content. The characteristics of S. miltiorrhiza seeds of rapidly absorbing a large amount of water were related to the structure of the mucilage cell layer. This structure is conducive to maintaining the seed moisture and resisting the drought
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